Collynn F Woeller1, Thomas H Thatcher, Daniel Van Twisk, Stephen J Pollock, Amanda Croasdell, Nina Kim, Philip K Hopke, Xiaoyan Xia, Juilee Thakar, Col Timothy M Mallon, Mark J Utell, Richard P Phipps. 1. Department of Environmental Medicine (Dr Woeller, Mr Pollock, Ms Croasdell, Ms Kim, Dr Utell, Dr Phipps), Department of Medicine (Drs Thatcher, Utell, and Phipps), Microbiology and Immunology, University of Rochester Medical Center (Mr Van Twisk, Drs Thakar and Phipps); Center for Air Resources Engineering and Science, Clarkson University, Potsdam, New York (Drs Hopke and Xia); and Professor, Department of Preventive Medicine and Biostatistics, Uniformed Services University, Bethesda, MD 20814 (Dr Mallon).
Abstract
OBJECTIVE: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs). METHODS: MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy. RESULTS: We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered. CONCLUSION: We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
OBJECTIVE: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs). METHODS: MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy. RESULTS: We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered. CONCLUSION: We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
Authors: Carl F Schaefer; Kira Anthony; Shiva Krupa; Jeffrey Buchoff; Matthew Day; Timo Hannay; Kenneth H Buetow Journal: Nucleic Acids Res Date: 2008-10-02 Impact factor: 16.971
Authors: Young-Mi Go; Matthew R Smith; Douglas I Walker; Karan Uppal; Patricia Rohrbeck; Pamela L Krahl; Philip K Hopke; Mark J Utell; Timothy M Mallon; Dean P Jones Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Atif Khan; Thomas H Thatcher; Collynn F Woeller; Patricia J Sime; Richard P Phipps; Philip K Hopke; Mark J Utell; Pamela L Krahl; Timothy M Mallon; Juilee Thakar Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Thomas H Thatcher; Collynn F Woeller; Juilee Thakar; Atif Khan; Philip K Hopke; Matthew Ryan Smith; Karan Uppal; Douglas I Walker; Young-Mi Go; Dean P Jones; Pamela L Krahl; Timothy M Mallon; Patricia J Sime; Richard P Phipps; Mark J Utell Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Juilee Thakar; Thomas H Thatcher; Matthew Ryan Smith; Collynn F Woeller; Douglas I Walker; Mark J Utell; Philip K Hopke; Timothy M Mallon; Pamela L Krahl; Patricia Rohrbeck; Young-Mi Go; Dean P Jones; Karan Uppal Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Matthew Ryan Smith; Douglas I Walker; Karan Uppal; Mark J Utell; Philip K Hopke; Timothy M Mallon; Pamela L Krahl; Patricia Rohrbeck; Young-Mi Go; Dean P Jones Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Collynn F Woeller; Thomas H Thatcher; Juilee Thakar; Adam Cornwell; Matthew R Smith; Dean P Jones; Philip K Hopke; Patricia J Sime; Pamela Krahl; Timothy M Mallon; Richard P Phipps; Mark J Utell Journal: J Occup Environ Med Date: 2019-12 Impact factor: 2.162
Authors: Vardaan Gupta; Christine L Hammond; Elisa Roztocil; Mithra O Gonzalez; Steven E Feldon; Collynn F Woeller Journal: Surv Ophthalmol Date: 2021-09-04 Impact factor: 6.197
Authors: Xiaoyan Xia; Alesia Carroll-Haddad; Nicole Brown; Mark J Utell; Col Timothy M Mallon; Philip K Hopke Journal: J Occup Environ Med Date: 2016-08 Impact factor: 2.162