Ivan Sabol1, Jana Smahelova2, Jan Klozar3, Marinka Mravak-Stipetic4, Tarik Gheit5, Massimo Tommasino5, Magdalena Grce6, Ruth Tachezy7. 1. Department of Immunology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic; Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia. 2. Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Czech Republic. 3. Department of Otorhinolaryngology and Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague, Motol University Hospital, Prague, Czech Republic. 4. Department of Oral Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia. 5. Infections and Cancer Biology Group, International Agency for Research on Cancer - World Health Organization, Lyon, France. 6. Department of Molecular Medicine, Ruđer Bošković Institute, Zagreb, Croatia. 7. Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Czech Republic. Electronic address: Ruth.Tachezy@uhkt.cz.
Abstract
BACKGROUND: Human papillomaviruses (HPV) are a heterogeneous group of viruses classified into five genera. The beta-HPV type (beta-PV) infection is very common but mostly asymptomatic in immunocompetent individuals. However, beta-PVs play a role in Epidermodysplasia verruciformis and possibly in non-melanoma skin cancer. Head and neck cancer (HNC) is a common cancer type worldwide and high-risk alpha-PV involvement in HNC has been extensively studied but beta-PV types have rarely been the focus of such studies. OBJECTIVES: To evaluate the prevalence of beta-PV types in HNC, subjects with non-malignant or potentially pre-malignant oral lesions, and healthy controls. STUDY DESIGN: The frequency of different beta-PVs in samples from oral (n=35) and oropharyngeal (n=35) cancer patients, gender- and age-matched healthy controls (n=70), and subjects with various non-malignant or potentially pre-malignant oral lesions (n=102) was assessed by a highly sensitive, bead-based, multiplex genotyping assay. RESULTS: Overall, 54.8% of all tested samples contained at least one beta-PV type. Even though the correlation between types found in lavage and tissue specimens from cancer patients was low, there was a large statistically significant difference between oropharyngeal cancer patients and matched controls for HPV5 (P=0.003; OR=15.58) and between both oral (P=0.026; OR=5.7) and oropharyngeal cancer patients (P=0.002; OR=25.5) and controls for HPV122. In addition, there was no correlation between the prevalence of alpha and beta-PVs in the study patients. CONCLUSION: The study provides new data on the prevalence of beta-PVs in HNC. HPV5 was found significantly associated with HNC as already observed by other studies. Additionally, the significant association of HPV122 with HNC might warrant further study as this type has not been extensively studied so far.
BACKGROUND: Human papillomaviruses (HPV) are a heterogeneous group of viruses classified into five genera. The beta-HPV type (beta-PV) infection is very common but mostly asymptomatic in immunocompetent individuals. However, beta-PVs play a role in Epidermodysplasia verruciformis and possibly in non-melanoma skin cancer. Head and neck cancer (HNC) is a common cancer type worldwide and high-risk alpha-PV involvement in HNC has been extensively studied but beta-PV types have rarely been the focus of such studies. OBJECTIVES: To evaluate the prevalence of beta-PV types in HNC, subjects with non-malignant or potentially pre-malignant oral lesions, and healthy controls. STUDY DESIGN: The frequency of different beta-PVs in samples from oral (n=35) and oropharyngeal (n=35) cancer patients, gender- and age-matched healthy controls (n=70), and subjects with various non-malignant or potentially pre-malignant oral lesions (n=102) was assessed by a highly sensitive, bead-based, multiplex genotyping assay. RESULTS: Overall, 54.8% of all tested samples contained at least one beta-PV type. Even though the correlation between types found in lavage and tissue specimens from cancer patients was low, there was a large statistically significant difference between oropharyngeal cancer patients and matched controls for HPV5 (P=0.003; OR=15.58) and between both oral (P=0.026; OR=5.7) and oropharyngeal cancer patients (P=0.002; OR=25.5) and controls for HPV122. In addition, there was no correlation between the prevalence of alpha and beta-PVs in the study patients. CONCLUSION: The study provides new data on the prevalence of beta-PVs in HNC. HPV5 was found significantly associated with HNC as already observed by other studies. Additionally, the significant association of HPV122 with HNC might warrant further study as this type has not been extensively studied so far.
Authors: Rachel L Winer; Tarik Gheit; Stephen Cherne; John Lin; Joshua E Stern; Mario Poljak; Qinghua Feng; Massimo Tommasino Journal: Papillomavirus Res Date: 2017-11-10
Authors: María Guadalupe Flores-Miramontes; Dominik Olszewski; Cristina Artaza-Irigaray; Anouk Willemsen; Ignacio G Bravo; Verónica Vallejo-Ruiz; Yelda Aurora Leal-Herrera; Patricia Piña-Sánchez; Andrea Molina-Pineda; Juan Carlos Cantón-Romero; María Guadalupe Martínez-Silva; Luis Felipe Jave-Suárez; Adriana Aguilar-Lemarroy Journal: Front Cell Infect Microbiol Date: 2020-06-09 Impact factor: 5.293
Authors: P Di Bonito; L Galati; A Focà; M Brambilla; C Bisaglia; G Bonanno Ferraro; P Mancini; M Iaconelli; C Veneri; G La Rosa Journal: J Appl Microbiol Date: 2019-07-12 Impact factor: 3.772