| Literature DB >> 27497994 |
Martin Roursgaard1, Kristina Bram Knudsen2, Helle Northeved3, Mikael Persson3, Tina Christensen3, Pramod E K Kumar4, Anders Permin5, Thomas L Andresen4, Torben Gjetting4, Jens Lykkesfeldt6, Lise K Vesterdal6, Steffen Loft6, Peter Møller6.
Abstract
The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.Entities:
Keywords: Comet assay; Drug delivery systems; Hepatocytes; In vitro toxicology; Liposomes; Lung epithelial cells; Micelles; Nanomedicine
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Year: 2016 PMID： 27497994 DOI： 10.1016/j.tiv.2016.08.002
Source DB: PubMed Journal: Toxicol In Vitro ISSN： 0887-2333 Impact factor: 3.500