Numan Kokten1, Oguz Kadir Egilmez2, A Isin Dogan Ekici3, M Tayyar Kalcioglu4, Muhammet Tekin1, Erdem Yesilada5. 1. Istanbul Medeniyet University, Faculty of Medicine, Göztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. 2. Malkara State Hospital, ENT Clinic, Tekirdağ, Turkey. 3. Yeditepe University, Faculty of Medicine, Department of Pathology, Istanbul, Turkey. 4. Istanbul Medeniyet University, Faculty of Medicine, Göztepe Training and Research Hospital, Department of Otorhinolaryngology, Istanbul, Turkey. Electronic address: mtkalcioglu@hotmail.com. 5. Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy, Istanbul, Turkey.
Abstract
OBJECTIVES: In this study, our aim was to identify the possible effects of Nigella sativa L. (NS) [blackcumin] seed oil on the prevention of experimentally induced myringosclerosis (MS). MATERIALS AND METHODS: Fourteen Guinea pigs were used and they were divided into three groups. Tympanic membranes (TM) of all animals were perforated and then group I was treated with saline soaked gel foams as a control group, group II was treated with 0.5 ml NS oil soaked gel foams at 0, 24 and 48 h and group III was treated with 5 ml NS oil orally at 0, 24, 48, 72 and 120 h. After 15 days, all animals were euthanized. Tympanic membranes were evaluated macroscopically and histopathologically. RESULTS: Groups I showed extensive myringosclerosis in contrast to those of Groups II and III which had significantly less changes (p < 0.05). The fibrosis and inflammation in the lamina propria of the tympanic membranes of Groups I was found to be significantly more pronounced (p < 0.05). The tympanic membranes were found to be significantly thinner in Groups II and III when compared with Groups I (p < 0.05). CONCLUSIONS: The results of this study suggested that topical or oral administration of NS oil supressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the Guinea pigs. For providing further evidence to use plant extracts as antioxidant and antiinflammatory therapy after myringotomy or ventilation tube insertion, further clinical studies with larger population will be essential.
OBJECTIVES: In this study, our aim was to identify the possible effects of Nigella sativa L. (NS) [blackcumin] seed oil on the prevention of experimentally induced myringosclerosis (MS). MATERIALS AND METHODS: Fourteen Guinea pigs were used and they were divided into three groups. Tympanic membranes (TM) of all animals were perforated and then group I was treated with saline soaked gel foams as a control group, group II was treated with 0.5 ml NS oil soaked gel foams at 0, 24 and 48 h and group III was treated with 5 ml NS oil orally at 0, 24, 48, 72 and 120 h. After 15 days, all animals were euthanized. Tympanic membranes were evaluated macroscopically and histopathologically. RESULTS: Groups I showed extensive myringosclerosis in contrast to those of Groups II and III which had significantly less changes (p < 0.05). The fibrosis and inflammation in the lamina propria of the tympanic membranes of Groups I was found to be significantly more pronounced (p < 0.05). The tympanic membranes were found to be significantly thinner in Groups II and III when compared with Groups I (p < 0.05). CONCLUSIONS: The results of this study suggested that topical or oral administration of NS oil supressed the inflammation and fibroblastic activity in the lamina propria of the myringotomized TMs of the Guinea pigs. For providing further evidence to use plant extracts as antioxidant and antiinflammatory therapy after myringotomy or ventilation tube insertion, further clinical studies with larger population will be essential.
Authors: Numan Kökten; Oğuz Kadir Eğilmez; Murat Erinç; A Işın Doğan Ekici; Serkan Şerifler; Erdem Yeşilada; Mahmut Tayyar Kalcıoğlu Journal: J Int Adv Otol Date: 2020-12 Impact factor: 1.017
Authors: Oğuz Kadir Eğilmez; Mehmet Güven; Sena Genç Elden; Özcan Budak; Hüseyin Çakıroğlu; Halil Elden; Ebru Mihriban Güven Journal: Turk Arch Otorhinolaryngol Date: 2021-07-30