Onur Beden1, Ender Senol1, Sevcan Atay2, Handan Ak2, Ayse Ender Altintoprak3, Guclu Selahattin Kiyan4, Buket Petin3, Utku Yaman3, Hikmet Hakan Aydin5. 1. Ege University, School of Medicine, Department of Forensic Medicine, Bornova, Izmir 35100, Turkey. 2. Ege University, School of Medicine, Department of Medical Biochemistry, Bornova, Izmir 35100, Turkey. 3. Ege University, School of Medicine, Department of Psychiatry, Bornova, Izmir 35100, Turkey. 4. Ege University, School of Medicine, Department of Emergency Medicine, Bornova, Izmir 35100, Turkey. 5. Ege University, School of Medicine, Department of Medical Biochemistry, Bornova, Izmir 35100, Turkey. Electronic address: hhakan.aydin@gmail.com.
Abstract
BACKGROUND: Serotonergic dysfunction is implicated in depression, psychiatric disorders and suicidal behaviors. The first and rate-limiting step in the synthesis of serotonin is catalyzed by tryptophan hydroxylase (TPH) which is encoded by TPH1 and THP2 genes. Genetic association studies have revealed contradictory results about the effect of the TPH1 A218C (rs1800532) polymorphism on suicidal behavior in different populations. MATERIAL AND METHOD: In this study, we investigated A218C polymorphism in 109 suicide attempters and 98 healthy controls. Socio-demographic characteristics of participants were obtained through questionnaire. DNA was extracted from peripheral blood and genotyping was performed by Real Time PCR. Fisher's exact test was used to evaluate the significance of the difference among the independent variables. Hardy-Weinberg equilibrium was tested using Pearson's goodness-of-fit chi-squared test. RESULTS: The frequency of A allele was significantly higher in suicide attempters than controls (46.33% vs. 35.71%, p=0.0357). However, there were no differences in genotype frequencies of this locus between participants having attempted suicide and controls (p>0.05). Among males, frequencies of CC genotype and C allele were found to be significantly higher in controls (p=0.0125, p=0.0298). With regard to the female subjects and female controls, no significant association was detected between suicidal behavior and genotype/allele frequencies (p>0.05). CONCLUSION: Our results provide evidence that A allele of TPH1 A218C polymorphism may be associated with suicidal behavior in Turkish population.
BACKGROUND: Serotonergic dysfunction is implicated in depression, psychiatric disorders and suicidal behaviors. The first and rate-limiting step in the synthesis of serotonin is catalyzed by tryptophan hydroxylase (TPH) which is encoded by TPH1 and THP2 genes. Genetic association studies have revealed contradictory results about the effect of the TPH1 A218C (rs1800532) polymorphism on suicidal behavior in different populations. MATERIAL AND METHOD: In this study, we investigated A218C polymorphism in 109 suicide attempters and 98 healthy controls. Socio-demographic characteristics of participants were obtained through questionnaire. DNA was extracted from peripheral blood and genotyping was performed by Real Time PCR. Fisher's exact test was used to evaluate the significance of the difference among the independent variables. Hardy-Weinberg equilibrium was tested using Pearson's goodness-of-fit chi-squared test. RESULTS: The frequency of A allele was significantly higher in suicide attempters than controls (46.33% vs. 35.71%, p=0.0357). However, there were no differences in genotype frequencies of this locus between participants having attempted suicide and controls (p>0.05). Among males, frequencies of CC genotype and C allele were found to be significantly higher in controls (p=0.0125, p=0.0298). With regard to the female subjects and female controls, no significant association was detected between suicidal behavior and genotype/allele frequencies (p>0.05). CONCLUSION: Our results provide evidence that A allele of TPH1 A218C polymorphism may be associated with suicidal behavior in Turkish population.
Authors: Gabriel Molina-Guzman; Thelma Beatriz González-Castro; Yazmín Hernández Díaz; Carlos Alfonso Tovilla-Zárate; Isela E Juárez-Rojop; Crystell Guadalupe Guzmán-Priego; Alma Genis; Sherezada Pool García; María Lilia López-Narvaez; José Manuel Rodriguez-Perez Journal: Neuropsychiatr Dis Treat Date: 2017-02-22 Impact factor: 2.570
Authors: Jadwiga Snarska; Ewa Fiedorowicz; Dominika Rozmus; Konrad Wroński; Maria Latacz; Natalia Kordulewska; Janusz Płomiński; Roman Grzybowski; Huub F J Savelkoul; Elżbieta Kostyra; Anna Cieślińska Journal: BMC Gastroenterol Date: 2021-11-12 Impact factor: 3.067
Authors: Alma Delia Genis-Mendoza; Yazmín Hernández-Díaz; Thelma Beatriz González-Castro; Carlos Alfonso Tovilla-Zárate; Rosa Giannina Castillo-Avila; María Lilia López-Narváez; Miguel Ángel Ramos-Méndez; Humberto Nicolini Journal: Front Psychiatry Date: 2022-07-19 Impact factor: 5.435