Laura Casarrubios1, María Concepción Matesanz2, Sandra Sánchez-Salcedo3, Daniel Arcos4, María Vallet-Regí5, María Teresa Portolés6. 1. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain; Instituto de Investigación Sanitaria San Carlos IdISSC, Spain. Electronic address: arualanilom@hotmail.com. 2. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain. Electronic address: conchitamatesanz@hotmail.com. 3. Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense de Madrid, Instituto de Investigación Hospital 12 de Octubre i+12, Spain; Networking Research Center on Bioengineering/Biomaterials and Nanomedicine, CIBER-BBN, Spain. Electronic address: sansanch@ucm.es. 4. Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense de Madrid, Instituto de Investigación Hospital 12 de Octubre i+12, Spain; Networking Research Center on Bioengineering/Biomaterials and Nanomedicine, CIBER-BBN, Spain. Electronic address: arcosd@ucm.es. 5. Department of Inorganic and Bioinorganic Chemistry, Faculty of Pharmacy, Universidad Complutense de Madrid, Instituto de Investigación Hospital 12 de Octubre i+12, Spain; Networking Research Center on Bioengineering/Biomaterials and Nanomedicine, CIBER-BBN, Spain. Electronic address: vallet@ucm.es. 6. Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense de Madrid, Spain; Instituto de Investigación Sanitaria San Carlos IdISSC, Spain. Electronic address: portoles@quim.ucm.es.
Abstract
HYPOTHESIS: Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200°C) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700°C) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two main cell types involved in bone remodelling) to silicon substituted hydroxyapatite. EXPERIMENTS: Saos-2 osteoblasts and osteoclast-like cells (differentiated from RAW-264.7 macrophages) have been cultured on the surface of nano-SiHA and SiHA disks and different cell parameters have been evaluated: cell adhesion, proliferation, viability, intracellular content of reactive oxygen species, cell cycle phases, apoptosis, cell morphology, osteoclast-like cell differentiation and resorptive activity. FINDINGS: This comparative in vitro study evidences that nanocrystallinity of SiHA affects the cell/biomaterial interface inducing bone cell apoptosis by loss of cell anchorage (anoikis), delaying osteoclast-like cell differentiation and decreasing the resorptive activity of this cell type. These results suggest the potential use of nano-SiHA biomaterial for preventing bone resorption in treatment of osteoporotic bone.
HYPOTHESIS: Silicon substituted hydroxyapatites (SiHA) are highly crystalline bioceramics treated at high temperatures (about 1200°C) which have been approved for clinical use with spinal, orthopedic, periodontal, oral and craniomaxillofacial applications. The preparation of SiHA with lower temperature methods (about 700°C) provides nanocrystalline SiHA (nano-SiHA) with enhanced bioreactivity due to higher surface area and smaller crystal size. The aim of this study has been to know the nanocrystallinity effects on the response of both osteoblasts and osteoclasts (the two main cell types involved in bone remodelling) to silicon substituted hydroxyapatite. EXPERIMENTS: Saos-2 osteoblasts and osteoclast-like cells (differentiated from RAW-264.7 macrophages) have been cultured on the surface of nano-SiHA and SiHA disks and different cell parameters have been evaluated: cell adhesion, proliferation, viability, intracellular content of reactive oxygen species, cell cycle phases, apoptosis, cell morphology, osteoclast-like cell differentiation and resorptive activity. FINDINGS: This comparative in vitro study evidences that nanocrystallinity of SiHA affects the cell/biomaterial interface inducing bone cell apoptosis by loss of cell anchorage (anoikis), delaying osteoclast-like cell differentiation and decreasing the resorptive activity of this cell type. These results suggest the potential use of nano-SiHA biomaterial for preventing bone resorption in treatment of osteoporotic bone.
Authors: Sandra Janeth Gutiérrez-Prieto; Luis F Fonseca; Luis Gonzalo Sequeda-Castañeda; Kelly J Díaz; Linet Y Castañeda; José A Leyva-Rojas; Juan Carlos Salcedo-Reyes; Adriana P Acosta Journal: Int J Dent Date: 2019-12-05