M Grąt1, K M Wronka2, M Krasnodębski2, Ł Masior2, Z Lewandowski3, I Kosińska4, K Grąt5, J Stypułkowski2, S Rejowski2, M Wasilewicz2, M Gałęcka6, P Szachta6, M Krawczyk2. 1. Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland. Electronic address: michal.grat@gmail.com. 2. Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland. 3. Department of Epidemiology, Medical University of Warsaw, Warsaw, Poland. 4. Department of Preventive Medicine and Hygiene, Medical University of Warsaw, Warsaw, Poland. 5. Second Department of Clinical Radiology, Medical University of Warsaw, Warsaw, Poland. 6. Institute of Microecology, Poznań, Poland.
Abstract
BACKGROUND: Changes within the gut microbiota contribute to the progression of chronic liver diseases. According to the results of several studies performed in animal models, gut dysbiosis plays an important role in hepatocarcinogenesis. The aim of this study was to explore the characteristics of gut microbiota associated with the presence of hepatocellular cancer (HCC) in patients with cirrhosis of the liver undergoing liver transplantation. METHODS: A total of 15 patients with HCC and 15 non-HCC patients matched according to etiology of cirrhosis and Model for End-Stage Liver Disease (MELD) scores who underwent liver transplantations between 2012 and 2014 were included. Analysis of their gut microbial profile was based on prospectively collected stool samples from the pretransplant period. RESULTS: Patients with and without HCC were similar with respect to age (P = .506), sex (P = .700), hepatitis C virus (P > .999) and hepatitis B virus (P = .715) infection status, alcoholic liver disease (P > .999), and MELD score (P = .337). Notably, the presence of HCC was associated with significantly increased fecal counts of Escherichia coli (P = .025). Prediction of HCC presence based on E coli counts was associated with the area under the receiver-operating curve of 0.742 (95% confidence interval, 0.564-0.920), with the optimal cutoff on the level of 17.728 (natural logarithm of colony-forming units per 1 g of feces). Sensitivity and specificity rates for the established cutoff were 66.7% and 73.3%, respectively. CONCLUSIONS: The profile of gut microbiota associated with the presence of HCC in cirrhotic patients is characterized by increased fecal counts of E coli. Therefore, intestinal overgrowth of E coli may contribute to the process of hepatocarcinogenesis.
BACKGROUND: Changes within the gut microbiota contribute to the progression of chronic liver diseases. According to the results of several studies performed in animal models, gut dysbiosis plays an important role in hepatocarcinogenesis. The aim of this study was to explore the characteristics of gut microbiota associated with the presence of hepatocellular cancer (HCC) in patients with cirrhosis of the liver undergoing liver transplantation. METHODS: A total of 15 patients with HCC and 15 non-HCC patients matched according to etiology of cirrhosis and Model for End-Stage Liver Disease (MELD) scores who underwent liver transplantations between 2012 and 2014 were included. Analysis of their gut microbial profile was based on prospectively collected stool samples from the pretransplant period. RESULTS:Patients with and without HCC were similar with respect to age (P = .506), sex (P = .700), hepatitis C virus (P > .999) and hepatitis B virus (P = .715) infection status, alcoholic liver disease (P > .999), and MELD score (P = .337). Notably, the presence of HCC was associated with significantly increased fecal counts of Escherichia coli (P = .025). Prediction of HCC presence based on E coli counts was associated with the area under the receiver-operating curve of 0.742 (95% confidence interval, 0.564-0.920), with the optimal cutoff on the level of 17.728 (natural logarithm of colony-forming units per 1 g of feces). Sensitivity and specificity rates for the established cutoff were 66.7% and 73.3%, respectively. CONCLUSIONS: The profile of gut microbiota associated with the presence of HCC in cirrhoticpatients is characterized by increased fecal counts of E coli. Therefore, intestinal overgrowth of E coli may contribute to the process of hepatocarcinogenesis.
Authors: Marco Sanduzzi Zamparelli; Alba Rocco; Debora Compare; Gerardo Nardone Journal: United European Gastroenterol J Date: 2017-05-08 Impact factor: 4.623
Authors: M Olmedo; E Reigadas; M Valerio; S Vázquez-Cuesta; J A Pajares; A Matilla; P Muñoz; E Bouza Journal: Rev Esp Quimioter Date: 2019-03-08 Impact factor: 1.553
Authors: Vishal Singh; Beng San Yeoh; Benoit Chassaing; Xia Xiao; Piu Saha; Rodrigo Aguilera Olvera; John D Lapek; Limin Zhang; Wei-Bei Wang; Sijie Hao; Michael D Flythe; David J Gonzalez; Patrice D Cani; Jose R Conejo-Garcia; Na Xiong; Mary J Kennett; Bina Joe; Andrew D Patterson; Andrew T Gewirtz; Matam Vijay-Kumar Journal: Cell Date: 2018-10-18 Impact factor: 41.582