Ranjana Walker Minz1, Yashwant Kumar1, Biman Saikia1, Shashi Anand1, Subhash Varma2, Surjit Singh3. 1. a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India. 2. b Department of Internal Medicine , Post Graduate Institute of Medical Education & Research , Chandigarh , India. 3. c Department of Rheumatology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
Abstract
OBJECTIVES: Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty. METHODS: To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD. RESULTS: Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm. CONCLUSION: Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening.
OBJECTIVES: Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty. METHODS: To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD. RESULTS: Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm. CONCLUSION: Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening.
Authors: Vivek Vasdev; S K Patnaik; D S Bhakuni; K Shanmuganandan; A Bhayana; G Mullick; A Hegde; Ashwini Kumar; Abhishek Kumar; R Singh Journal: Med J Armed Forces India Date: 2020-07-09