Seshagiri Muttagi1, Jyothi Keralapura Subramanya2. 1. Professor, Department of Biochemistry, Coorg Institute of Dental Sciences, Virajpet, Coorg, and Rajiv Gandhi University of Health Sciences, Bangalore, India. Electronic address: seshim2000@gmail.com. 2. Professor, Department of Prosthodontics and Crown and Bridge and Implantology, Coorg Institute of Dental Sciences, Virajpet, Coorg, and Rajiv Gandhi University of Health Sciences, Bangalore, India.
Abstract
STATEMENT OF PROBLEM: The colonization of Candida albicans on soft liners causes the deterioration of material surface properties and denture stomatitis. PURPOSE: The purpose of this in vitro study was to investigate the effect of incorporating seed oil on the antifungal properties, surface roughness, wettability, weight changes, and glucose adsorption/absorption of a soft liner. MATERIAL AND METHODS: Centratherum anthelminticum, Ocimum sanctum Linn, and Linum usitatissimum seed oils were incorporated into a soft liner, and the diameter of the inhibition zone (DIZ) was calculated relative to the growth of C albicans at 24, 48, and 72 hours and 7 days. The effect of incorporating oil on wettability was evaluated with the contact angle analyzer, and effect on surface roughness was evaluated with scanning electron microscopy. Weight changes and absorption/adsorption of glucose to the soft liner were estimated. Data were analyzed by using paired Student t tests and 2-way ANOVA (α=.01). RESULTS: Soft liner with 800 μL each of C anthelminticum and O sanctum oils showed complete inhibition at 72 hours; the DIZ on day 7 were 31.66 ±1.20 mm and 29.66 ±1.46 mm, respectively. The change in weight between time intervals differed significantly between conditions (P<.01). The addition of oils decreased the surface roughness and improved wettability significantly for O sanctum and C anthelminticum specimens (P<.01). At 72 hours, 5.5 mg of glucose was detected in the positive control, whereas no glucose was detected in the oil specimens. CONCLUSIONS: The addition of the seed oils of C anthelminticum and O sanctum to the soft liner significantly reduced the growth of C albicans, improved wettability, reduced surface roughness, and minimized the absorption and adsorption of glucose.
STATEMENT OF PROBLEM: The colonization of Candida albicans on soft liners causes the deterioration of material surface properties and denture stomatitis. PURPOSE: The purpose of this in vitro study was to investigate the effect of incorporating seed oil on the antifungal properties, surface roughness, wettability, weight changes, and glucose adsorption/absorption of a soft liner. MATERIAL AND METHODS:Centratherum anthelminticum, Ocimum sanctum Linn, and Linum usitatissimumseed oils were incorporated into a soft liner, and the diameter of the inhibition zone (DIZ) was calculated relative to the growth of C albicans at 24, 48, and 72 hours and 7 days. The effect of incorporating oil on wettability was evaluated with the contact angle analyzer, and effect on surface roughness was evaluated with scanning electron microscopy. Weight changes and absorption/adsorption of glucose to the soft liner were estimated. Data were analyzed by using paired Student t tests and 2-way ANOVA (α=.01). RESULTS: Soft liner with 800 μL each of C anthelminticum and O sanctum oils showed complete inhibition at 72 hours; the DIZ on day 7 were 31.66 ±1.20 mm and 29.66 ±1.46 mm, respectively. The change in weight between time intervals differed significantly between conditions (P<.01). The addition of oils decreased the surface roughness and improved wettability significantly for O sanctum and C anthelminticum specimens (P<.01). At 72 hours, 5.5 mg of glucose was detected in the positive control, whereas no glucose was detected in the oil specimens. CONCLUSIONS: The addition of the seed oils of C anthelminticum and O sanctum to the soft liner significantly reduced the growth of C albicans, improved wettability, reduced surface roughness, and minimized the absorption and adsorption of glucose.