Xiaoyun Wu1,2, Huiyan Kang2, Xuemin Liu2, Jin Gao3,4, Kuijun Zhao1, Zhijie Ma5. 1. Department of pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China. 2. Department of Technology, Beijing JingMeng Stem Cell Technology. Co. Ltd., Beijing, China. 3. Beijing Institute of Life Science Translational Medicine Research Center, Beijing, China. 4. Center for Tissue Engineering and Technology of Inner Mongolia, Hohhot, Inner Mongolia, China. 5. Department of pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China. 13811647091@163.com.
Abstract
OBJECTIVES: Umbilical cord mesenchymal stromal cells (UCMSCs) can be considered to become a new gold standard for MSC-based therapies. A serum and xeno-free, chemically defined and no-plate-coating-based culture system will greatly facilitate development of robust, clinically acceptable bioprocesses for reproducibly generating quality-assured UCMSCs. MATERIALS AND METHODS: In this study, we report for the first time, such a serum-free, xeno-free, completely chemically defined and no-plate-coating-based culture system for the isolation and expansion of UCMSCs, whose biological characteristics were evaluated and compared with serum-containing medium (SCM) methods. RESULTS: This culture system not only supported UCMSC primary cultures but also allowed for their expansion at low seeding density. Compared to SCM, UCMSCs in SFM exhibited (i) higher proliferative and colony-forming capacities; (ii) distinctly different morphologies; (iii) similar phenotype; (iv) similar pluripotency-associated marker expression; (v) superior osteogenic, but reduced adipogenic differentiation capacitities. In addition, UCMSCs cultured in SFM retained similar immunomodulatory properties to those in SCM. CONCLUSIONS: Our findings demonstrate the feasibility of isolating and expanding UCMSCs in a completely serum-free, xeno-free, chemically defined and no-plate-coating-based culture system and represent an important step forward for development of robust, clinically acceptable bioprocesses for UCMSCs. Further, this provides a superior study platform for UCMSCs biology in a controlled environment.
OBJECTIVES: Umbilical cord mesenchymal stromal cells (UCMSCs) can be considered to become a new gold standard for MSC-based therapies. A serum and xeno-free, chemically defined and no-plate-coating-based culture system will greatly facilitate development of robust, clinically acceptable bioprocesses for reproducibly generating quality-assured UCMSCs. MATERIALS AND METHODS: In this study, we report for the first time, such a serum-free, xeno-free, completely chemically defined and no-plate-coating-based culture system for the isolation and expansion of UCMSCs, whose biological characteristics were evaluated and compared with serum-containing medium (SCM) methods. RESULTS: This culture system not only supported UCMSC primary cultures but also allowed for their expansion at low seeding density. Compared to SCM, UCMSCs in SFM exhibited (i) higher proliferative and colony-forming capacities; (ii) distinctly different morphologies; (iii) similar phenotype; (iv) similar pluripotency-associated marker expression; (v) superior osteogenic, but reduced adipogenic differentiation capacitities. In addition, UCMSCs cultured in SFM retained similar immunomodulatory properties to those in SCM. CONCLUSIONS: Our findings demonstrate the feasibility of isolating and expanding UCMSCs in a completely serum-free, xeno-free, chemically defined and no-plate-coating-based culture system and represent an important step forward for development of robust, clinically acceptable bioprocesses for UCMSCs. Further, this provides a superior study platform for UCMSCs biology in a controlled environment.
Authors: Enrico Pierantozzi; Barbara Gava; Ivana Manini; Franco Roviello; Giuseppe Marotta; Mario Chiavarelli; Vincenzo Sorrentino Journal: Stem Cells Dev Date: 2010-10-29 Impact factor: 3.272
Authors: Sanjay Gottipamula; Manjunatha S Muttigi; S Chaansa; K M Ashwin; Nancy Priya; Udaykumar Kolkundkar; Swathi SundarRaj; Anish Sen Majumdar; Raviraja N Seetharam Journal: J Tissue Eng Regen Med Date: 2013-03-12 Impact factor: 3.963