Haifang Zhang1,2,3,4, Yanwei Jia1,2,3,4, Xiaofang Xie1,2,3,4, Min Wang1,2,3,4, Yi Zheng1,2,3,4, Shungao Xu1,2,3,4, Wei Zhang1,2,3,4, Qiang Wang1,2,3,4, Xinxiang Huang1,2,3,4, Hong Du1,2,3,4. 1. Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. 2. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. 3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. 4. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.
Abstract
AIM: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella. MATERIALS & METHODS: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress. RESULTS: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains. CONCLUSION: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.
AIM: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella. MATERIALS & METHODS: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress. RESULTS: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains. CONCLUSION: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.
Authors: Haifang Zhang; Minhui Miao; Jieting Yan; Min Wang; Yi-Wei Tang; Barry N Kreiswirth; Xia Zhang; Liang Chen; Hong Du Journal: Oncotarget Date: 2017-11-20