Carlos Stambolsky1, Soledad Rodríguez-Benítez1, José Luis Gutiérrez-Pérez2, Daniel Torres-Lagares1, Jenifer Martín-González1, Juan José Segura-Egea3. 1. Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain. 2. Department of Stomatology, School of Dentistry, Virgen Macarena University Hospital, Andalusian Health Service, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain. 3. Department of Stomatology, School of Dentistry, University of Sevilla, C/Avicena s/n, 41009 Sevilla, Spain. Electronic address: segurajj@us.es.
Abstract
INTRODUCTION: This study evaluates histologically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP). METHODS: Forty double-rooted immature premolar teeth from 4 female Beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP. Four teeth were left untreated and assigned to the positive control group. The last 28 teeth were randomly assigned into four experimental groups of 8 teeth, each one treated with a different treatment protocol: A1, sodium hypochlorite (SH)+blood clot (BC); A2, SH+platelet-rich plasma (PRP); B1, SH+modified tri-antibiotic paste (mTAP)+BC; B2, SH+mTAP+PRP. The animals were sacrificed, histologic sections were prepared and three parameters were assessed: (1) presence or absence of new hard tissue on the internal root dentinal walls, (2) presence or absence of continued apical closure, and (3) presence or absence of vital tissue within the canal space. RESULTS: Significant differences (p<0.05) between the four experimental groups were evident in the percentage of teeth showing histological apical closure (34.5%) and vital tissue within the canal space (68.8%). Group B2 showed the maximal improvement in the three variables assessed (p<0.05). Group A1 showed the minimum percentages in the three parameters assessed (p<0.05). CONCLUSIONS: These results suggest that an intracanal dressing of mTAP, and the use of PRP as scaffold, improves the success rate of the revascularization procedure.
INTRODUCTION: This study evaluates histologically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP). METHODS: Forty double-rooted immature premolar teeth from 4 female Beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP. Four teeth were left untreated and assigned to the positive control group. The last 28 teeth were randomly assigned into four experimental groups of 8 teeth, each one treated with a different treatment protocol: A1, sodium hypochlorite (SH)+blood clot (BC); A2, SH+platelet-rich plasma (PRP); B1, SH+modified tri-antibiotic paste (mTAP)+BC; B2, SH+mTAP+PRP. The animals were sacrificed, histologic sections were prepared and three parameters were assessed: (1) presence or absence of new hard tissue on the internal root dentinal walls, (2) presence or absence of continued apical closure, and (3) presence or absence of vital tissue within the canal space. RESULTS: Significant differences (p<0.05) between the four experimental groups were evident in the percentage of teeth showing histological apical closure (34.5%) and vital tissue within the canal space (68.8%). Group B2 showed the maximal improvement in the three variables assessed (p<0.05). Group A1 showed the minimum percentages in the three parameters assessed (p<0.05). CONCLUSIONS: These results suggest that an intracanal dressing of mTAP, and the use of PRP as scaffold, improves the success rate of the revascularization procedure.
Authors: Mauricio Gonçalves da Costa Sousa; Gabriela Conceição de Almeida; Danilo César Martins Mota; Rosiane Andrade da Costa; Simoni Campos Dias; Samuel Nunes Limberger; Frank Ko; Li Ting Lin; Evan F Haney; Hashem Etayash; Beverlie Baquir; Michael J Trimble; Ya Shen; Zheng Su; Markus Haapasalo; Daniel Pletzer; Letícia Chaves de Souza; Gláucia Schuindt Teixeira; Renato M Silva; Robert E W Hancock; Octavio Luiz Franco; Taia Maria Berto Rezende Journal: Bioact Mater Date: 2022-02-01
Authors: Ahmed Sabry Ammar; Yasser Osman; Ahmed Taher Hendam; Mohammed Ahmed Hasen; Fatma Abdullah Al Rubaish; Danya Yaagoub Al Nujaidi; Faisal Mishal Al Abbas Journal: Asian J Neurosurg Date: 2017 Oct-Dec