Achilleas Papatheodorou1, Pierre Vanderzwalmen2, Yannis Panagiotidis3, Stamatios Petousis4, Giuseppe Gullo5, Evangelia Kasapi3, Maria Goudakou3, Nikos Prapas3, Kostas Zikopoulos6, Ioannis Georgiou6, Yannis Prapas3. 1. IAKENTRO Advanced Medical Center, Thessaloniki, Greece; Center for Reproductive Medicine, Obstetrics and Gynecology, Ioannina University, Ioannina, Greece. Electronic address: achilleas@iakentro.gr. 2. IVF Centers Prof Zech, Bregenz, Austria; Chirec, Brussels, Belgium. 3. IAKENTRO Advanced Medical Center, Thessaloniki, Greece. 4. IAKENTRO Advanced Medical Center, Thessaloniki, Greece; 3rd Department of Obstetrics and Gynaecology, Aristotle University, Thessaloniki, Greece. 5. Department of Obstetrics and Gynaecology, University of Messina, Messina, Italy. 6. Center for Reproductive Medicine, Obstetrics and Gynecology, Ioannina University, Ioannina, Greece.
Abstract
OBJECTIVE: To evaluate whether is possible to vitrify oocytes in an aseptic (hermetically closed) fashion and maintain clinical results comparable with those of fresh oocytes. DESIGN: Prospective, observational, cohort, noninferiority trial. SETTING: Private in vitro fertilization center. PATIENT(S): One hundred eighty-four recipients of donated vitrified oocytes. INTERVENTION(S): Closed system vitrification. MAIN OUTCOME MEASURE(S): Pregnancy rate per cycle and clinical pregnancy rate per cycle. RESULT(S): No statistically significant differences were observed between two groups regarding the pregnancy rate per cycle (63.1% vs. 60.9%) or the clinical pregnancy rate per cycle (55.4% vs. 58.7%). Biochemical pregnancy rate was statistically significantly higher in the fresh group (7.6% vs. 2.2%). The mean number of embryos transferred was similar (2.0 ± 0.0 vs. 1.97 ± 0.3). Concerning embryologic data, there were no statistically significant differences regarding the fertilization, cleavage, top quality day-3 embryo, or blastocyst rates, whereas the top quality blastocyst rate on day 5 was statistically significantly higher in the fresh oocyte group (31.7% vs. 26.1%). CONCLUSION(S): Aseptically (in a closed system) vitrified oocytes show similar clinical efficiency compared with their sibling fresh oocytes.
OBJECTIVE: To evaluate whether is possible to vitrify oocytes in an aseptic (hermetically closed) fashion and maintain clinical results comparable with those of fresh oocytes. DESIGN: Prospective, observational, cohort, noninferiority trial. SETTING: Private in vitro fertilization center. PATIENT(S): One hundred eighty-four recipients of donated vitrified oocytes. INTERVENTION(S): Closed system vitrification. MAIN OUTCOME MEASURE(S): Pregnancy rate per cycle and clinical pregnancy rate per cycle. RESULT(S): No statistically significant differences were observed between two groups regarding the pregnancy rate per cycle (63.1% vs. 60.9%) or the clinical pregnancy rate per cycle (55.4% vs. 58.7%). Biochemical pregnancy rate was statistically significantly higher in the fresh group (7.6% vs. 2.2%). The mean number of embryos transferred was similar (2.0 ± 0.0 vs. 1.97 ± 0.3). Concerning embryologic data, there were no statistically significant differences regarding the fertilization, cleavage, top quality day-3 embryo, or blastocyst rates, whereas the top quality blastocyst rate on day 5 was statistically significantly higher in the fresh oocyte group (31.7% vs. 26.1%). CONCLUSION(S): Aseptically (in a closed system) vitrified oocytes show similar clinical efficiency compared with their sibling fresh oocytes.
Authors: Gaspare Cucinella; Giuseppe Di Buono; Girolamo Geraci; Federica Ricupati; Giuseppe Gullo; Elisa Maienza; Giorgio Romano; Giulia Bonventre; Giuseppe Amato; Giorgio Romano; Salvatore Buscemi; Antonino Agrusa Journal: Front Surg Date: 2022-05-12