Nisha Susan Thomas1, Kiran George2, Sivaranjani Arivalagan1, Vijay Mani1, Aktarul Islam Siddique1, Nalini Namasivayam3. 1. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu, 608 002, India. 2. Department of Instrumentation Engineering, Bioengineering, Faculty of Engineering and Technology, Annamalai University, Annamalainagar, Chidambaram, 608002, Tamil Nadu, India. 3. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu, 608 002, India. nalininam@yahoo.com.
Abstract
PURPOSE: Troxerutin (TXER), a trihydroxyethylated derivative of the natural bioflavonoid rutin, abundantly found in tea, various fruits and vegetables, is known to exhibit ample pharmacological properties. In the present investigation, we examined the antineoplastic, therapeutic efficacy and furthermore the possible mechanisms of action of TXER against NAFLD/NASH progression to hepatocarcinogenesis. METHODS: The effect of TXER (12.5, 25 or 50 mg/kg b.w/day) was evaluated on the nitrosodiethylamine (NDEA) model of hepatocarcinogenesis in rats, after 16 weeks of oral treatment, with special focus on liver specific enzymes, xenobiotic metabolizing enzymes, antioxidant status, lipid peroxidation profile, DNA damage, fibrosis, cell proliferation and inflammatory status. RESULTS: Administration of TXER to hepatocellular carcinoma-bearing rats restored the enzyme activities and the hepatic architecture. Furthermore, TXER significantly curtailed NDEA-induced DNA damage, cell proliferation, inflammation, fibrosis and hepatic hyperplasia. CONCLUSION: This study provides the evidence that troxerutin exerts a significant therapeutic effect against liver cancer by modulating liver function enzymes, xenobiotic enzymes, oxidative damage, inhibiting cell proliferation, suppressing inflammatory response and induction of apoptosis.
PURPOSE:Troxerutin (TXER), a trihydroxyethylated derivative of the natural bioflavonoidrutin, abundantly found in tea, various fruits and vegetables, is known to exhibit ample pharmacological properties. In the present investigation, we examined the antineoplastic, therapeutic efficacy and furthermore the possible mechanisms of action of TXER against NAFLD/NASH progression to hepatocarcinogenesis. METHODS: The effect of TXER (12.5, 25 or 50 mg/kg b.w/day) was evaluated on the nitrosodiethylamine (NDEA) model of hepatocarcinogenesis in rats, after 16 weeks of oral treatment, with special focus on liver specific enzymes, xenobiotic metabolizing enzymes, antioxidant status, lipid peroxidation profile, DNA damage, fibrosis, cell proliferation and inflammatory status. RESULTS: Administration of TXER to hepatocellular carcinoma-bearing rats restored the enzyme activities and the hepatic architecture. Furthermore, TXER significantly curtailed NDEA-induced DNA damage, cell proliferation, inflammation, fibrosis and hepatic hyperplasia. CONCLUSION: This study provides the evidence that troxerutin exerts a significant therapeutic effect against liver cancer by modulating liver function enzymes, xenobiotic enzymes, oxidative damage, inhibiting cell proliferation, suppressing inflammatory response and induction of apoptosis.
Authors: Sanjay Kalra; Manoj Vithalani; Gurjeet Gulati; C M Kulkarni; Yogesh Kadam; James Pallivathukkal; Brahmananda Das; Rakesh Sahay; K D Modi Journal: J Assoc Physicians India Date: 2013-07
Authors: Laura J Niedernhofer; J Scott Daniels; Carol A Rouzer; Rachel E Greene; Lawrence J Marnett Journal: J Biol Chem Date: 2003-05-29 Impact factor: 5.157
Authors: João Antônio Leal de Miranda; Conceição da Silva Martins; Lázaro de Sousa Fideles; Maria Lucianny Lima Barbosa; João Erivan Façanha Barreto; Helder Bindá Pimenta; Francisco Orlando Rafael Freitas; Paulo Vitor de Souza Pimentel; Claudio Silva Teixeira; Ariel Gustavo Scafuri; Maria Claudia Dos Santos Luciano; Joabe Lima Araújo; Jefferson Almeida Rocha; Icaro Gusmão Pinto Vieira; Nágila Maria Pontes Silva Ricardo; Matheus da Silva Campelo; Maria Elenir Nobre Pinho Ribeiro; Gerly Anne de Castro Brito; Gilberto Santos Cerqueira Journal: Pharmaceuticals (Basel) Date: 2020-01-08