| Literature DB >> 27488184 |
Li-Hsuen Chen1,2, Wen-Lin Hsu2,3, Yen-Ju Tseng1,2, Dai-Wei Liu4,5, Ching-Feng Weng6.
Abstract
Entities:
Year: 2016 PMID: 27488184 PMCID: PMC4973097 DOI: 10.1186/s12885-016-2560-2
Source DB: PubMed Journal: BMC Cancer ISSN: 1471-2407 Impact factor: 4.430
Fig. 3Aberrant expression of DNMT 3B in HNSCC cell lines and knockdown of DNMT 3B in A253-5 reversed EMT marker genes. a DNMT 3B protein expression in HNSCC cell lines and its invasive subpopulations. b DNMTs and EMT marker genes protein expression in A253 and its invasive subpopulations (A253-3, A253-5) and A253-5 transfected with siRNA against DNMT 3B (A253-5 si). ** indicated p < 0.01 and *** indicated p < 0.001 as compared with A253-0. ### indicated p < 0.001 as compared with A253-5si
Fig. 5MiRNA 29b mimic targeted DNMT 3B and reversed EMT. a Q-PCR examination of miRNA 29b expression in A253 cells. * indicated p < 0.05 and ** indicated p < 0.01 as compared with A253-0. # indicated that there were no statistically significant between A253-5 and A253-5si (Knockdown of DNMT 3B). b Transfection of miRNA 29b mimic could downregulate DNMT 3B and reverse EMT marker genes in A253-5. c, d Migration and invasion assay. Knockdown DNMT 3B by small interfering RNA or mir-29b mimic could inhibit cell mobility. * indicated p < 0.05, ** indicated p < 0.01 and *** indicated p < 0.001 as compared with A253-5