| Literature DB >> 27485337 |
Ravi C Kalathur1, Marinela Panganiban2, Renato Bruni2.
Abstract
The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.Keywords: Bacmid; Baculovirus; Expression; High-throughput; Insect cell; Membrane protein; Nickel affinity chromatography; Purification; Sf9
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Year: 2016 PMID： 27485337 DOI： 10.1007/978-1-4939-3637-3_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745