| Literature DB >> 27485328 |
Federica Angius1, Oana Ilioaia1, Marc Uzan1, Bruno Miroux2,3.
Abstract
Functional and structural studies on membrane proteins are limited by the difficulty to produce them in large amount and in a functional state. In this review, we provide protocols to achieve high-level expression of membrane proteins in Escherichia coli. The T7 RNA polymerase-based expression system is presented in detail and protocols to assess and improve its efficiency are discussed. Protocols to isolate either membrane or inclusion bodies and to perform an initial qualitative test to assess the solubility of the recombinant protein are also included.Entities:
Keywords: E. coli; Production of recombinant proteins; T7 RNA polymerase
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Year: 2016 PMID： 27485328 DOI： 10.1007/978-1-4939-3637-3_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745