Jing Xing1,2, Gaoxin Zhou1,2, Chunlong Sun3, Huanqing Zhang4, Bo Chen1,2, Xi Zong5, Jin Cai5, Min Ji6,7. 1. School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China. 2. School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China. 3. College of Life Sciences, Binzhou University, Binzhou, 256600, China. 4. Chia-tai Tianqing Pharmaceutical Group Co. Ltd., No.699-8 Xuanwu Blvd., Nanjing, 210042, China. 5. School of Chemistry & Chemical Engineering, Southeast University, Nanjing, 210096, China. 6. School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China. seujimin@126.com. 7. School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China. seujimin@126.com.
Abstract
OBJECTIVE: To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells. RESULTS: A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells. CONCLUSION: IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.
OBJECTIVE: To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells. RESULTS: A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells. CONCLUSION: IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.
Entities:
Keywords:
A549 cells; Fluorescence imaging; Fluorescent dye; IR-793; Imaging of living cells; Indocyanine Green; Living cells