| Literature DB >> 27483206 |
Tatsuya Nishihara1, Hikari A I Yoshihara2, Hiroshi Nonaka1, Yoichi Takakusagi3, Fuminori Hyodo4, Kazuhiro Ichikawa3,4, Emine Can2, Jessica A M Bastiaansen2, Yuhei Takado2,5, Arnaud Comment6, Shinsuke Sando7.
Abstract
The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-(13) C]Gly, for monitoring GGT activity in vivo by hyperpolarized (HP) (13) C magnetic resonance (MR). The properties of γ-Glu-[1-(13) C]Gly are suitable for in vivo HP (13) C metabolic analysis since the chemical shift between γ-Glu-[1-(13) C]Gly and its metabolic product, [1-(13) C]Gly, is large (4.3 ppm) and the T1 of both compounds is relatively long (30 s and 45 s, respectively, in H2 O at 9.4 T). We also demonstrate that γ-Glu-[1-(13) C]Gly is highly sensitive to in vivo modulation of GGT activity induced by the inhibitor acivicin.Entities:
Keywords: NMR spectroscopy; biosensors; dynamic nuclear polarization; hyperpolarization; γ-glutamyl transpeptidase
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Year: 2016 PMID： 27483206 DOI： 10.1002/anie.201603731
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336