Development of surface curcumin nanoparticles modified with biological macromolecules for anti-tumor effects.

The rationale of this study was to improve the stability, cellular uptake, and evaluate the cytotoxicity of surface modified curcumin nanoparticles (CUR NP). CUR NP were surface modified with proteins (transferrin [Tf] and gelatin [GT]) by adsorption to improve their stability and targeting property. CUR NP were evaluated for stability, in vitro drug release, cellular uptake and cell cytotoxicity. The particle sizes of CUR NP were 153.2±56.4nm (CUR NP), 145.0±26.8nm (Tf-CUR NP), and 167.7±42.7nm (GT-CUR NP). The stabilities of Tf-CUR NP and GT-CUR NP were higher than that of CUR NP. Tf-CUR NP and GT-CUR NP showed faster drug release than those shown by CUR NP and CUR (pure) in pH 7.4 PBS and cell media (RPMI) for 36h. The cellular uptake and cytotoxicity of Tf- and GT-modified CUR NP were higher than those of CUR NP in MCF-7 and A549 cells. In conclusion, Tf-CUR NP and GT-CUR NP exhibited improved stability, enhanced cellular uptake, and stronger cytotoxicity.