Literature DB >> 27480714

Fluorescent Labeling of Yeast Cell Wall Components.

Hiroki Okada1, Yoshikazu Ohya1.   

Abstract

Yeast cells stained with a fluorescent dye that specifically binds to one of the cell wall components can be observed under a fluorescent microscope. Visualization of the components 1,3-β-glucan, mannoproteins, and/or chitin not only provides information concerning the cell wall, but also reveals clues about various cellular activities such as cell polarity, vesicular transport, establishment of budding pattern, apical and isotropic bud growth, and replicative cell age. This protocol describes a standard method for visualizing different components of the yeast cell wall.
© 2016 Cold Spring Harbor Laboratory Press.

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Year:  2016        PMID: 27480714     DOI: 10.1101/pdb.prot085241

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  7 in total

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2.  Optimised Extraction and Preliminary Characterisation of Mannoproteins from Non-Saccharomyces Wine Yeasts.

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Journal:  Foods       Date:  2021-04-22

3.  Coordinated Roles of the Putative Ceramide-Conjugation Protein, Cwh43, and a Mn2+-Transporting, P-Type ATPase, Pmr1, in Fission Yeast.

Authors:  Norihiko Nakazawa; Xingya Xu; Orie Arakawa; Mitsuhiro Yanagida
Journal:  G3 (Bethesda)       Date:  2019-08-08       Impact factor: 3.154

4.  Jerveratrum-Type Steroidal Alkaloids Inhibit β-1,6-Glucan Biosynthesis in Fungal Cell Walls.

Authors:  Karen Kubo; Kaori Itto-Nakama; Shinsuke Ohnuki; Yoko Yashiroda; Sheena C Li; Hiromi Kimura; Yumi Kawamura; Yasuhiro Shimamoto; Ken-Ichi Tominaga; Daisuke Yamanaka; Yoshiyuki Adachi; Shinichiro Takashima; Yoichi Noda; Charles Boone; Yoshikazu Ohya
Journal:  Microbiol Spectr       Date:  2022-01-12

5.  Fluconazole resistant Candida auris clinical isolates have increased levels of cell wall chitin and increased susceptibility to a glucosamine-6-phosphate synthase inhibitor.

Authors:  Garima Shahi; Mohit Kumar; Andrzej S Skwarecki; Matt Edmondson; Atanu Banerjee; Jane Usher; Neil A R Gow; Sławomir Milewski; Rajendra Prasad
Journal:  Cell Surf       Date:  2022-02-25

Review 6.  An important polysaccharide from fermentum.

Authors:  Bobo Lin; Gangliang Huang
Journal:  Food Chem X       Date:  2022-07-08

7.  Yeast species-specific, differential inhibition of β-1,3-glucan synthesis by poacic acid and caspofungin.

Authors:  Keunsook K Lee; Karen Kubo; Jehan Abdelmoneim Abdelaziz; Iain Cunningham; Alessandra de Silva Dantas; Xiaolin Chen; Hiroki Okada; Yoshikazu Ohya; Neil A R Gow
Journal:  Cell Surf       Date:  2018-09
  7 in total

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