Mauro Jorge Cabral-Castro1, Marta Guimarães Cavalcanti2, Regina Helena Saramago Peralta3, José Mauro Peralta4. 1. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil. 2. Departamento de Doenças Infecciosas e Parasitárias, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. 3. Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil. 4. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil. Electronic address: peralta@micro.ufrj.br.
Abstract
BACKGROUND: Arboviruses are important emerging viruses worldwide. The signs and symptoms of Zika virus (ZIKV) infection are similar to those presented by infections with dengue virus (DENV) and chikungunya virus (CHIKV). Furthermore, diagnosis of ZIKV infection is particularly challenging in dengue endemic regions and with co-circulation of DENV, CHIKV, and ZIKV, making diagnosis based solely on clinical and epidemiological data unreliable. As these three viral infections share similar clinical manifestations, differential diagnosis is crucial. OBJECTIVES: In this study, diagnoses of ZIKV, CHIKV and DENV infections were investigated in 30 patients with suspected dengue fever residing in the area of co-circulation of these three arboviruses. STUDY DESIGN: The study included whole blood and/or serum samples obtained from 30 patients with suspected dengue fever. All patients were tested for DENV infection as well as for CHIKV and ZIKV infections. Assays for detecting anti-DENV IgM and DENV RNA by semi-nested RT-PCR and ZIKV and CHIKV RNA by real-time RT-PCR were performed. RESULTS: DENV RNA was not detectable in any of the clinical samples, whereas ZIKV RNA was detectable in 17 samples (56.7%). Co-infection by ZIKV and CHIKV was documented in one case. Of the 17 ZIKV-positive individuals, 8 showed reactivity for anti-DENV IgM, which suggested recent DENV infection, cross-reactivity or co-infection. CONCLUSION: Our findings confirm that accurate laboratory testing is of paramount importance for differential diagnosis in areas of simultaneous transmission of different arboviruses with similar clinical presentations.
BACKGROUND: Arboviruses are important emerging viruses worldwide. The signs and symptoms of Zika virus (ZIKV) infection are similar to those presented by infections with dengue virus (DENV) and chikungunya virus (CHIKV). Furthermore, diagnosis of ZIKV infection is particularly challenging in dengue endemic regions and with co-circulation of DENV, CHIKV, and ZIKV, making diagnosis based solely on clinical and epidemiological data unreliable. As these three viral infections share similar clinical manifestations, differential diagnosis is crucial. OBJECTIVES: In this study, diagnoses of ZIKV, CHIKV and DENV infections were investigated in 30 patients with suspected dengue fever residing in the area of co-circulation of these three arboviruses. STUDY DESIGN: The study included whole blood and/or serum samples obtained from 30 patients with suspected dengue fever. All patients were tested for DENVinfection as well as for CHIKV and ZIKV infections. Assays for detecting anti-DENV IgM and DENV RNA by semi-nested RT-PCR and ZIKV and CHIKV RNA by real-time RT-PCR were performed. RESULTS:DENV RNA was not detectable in any of the clinical samples, whereas ZIKV RNA was detectable in 17 samples (56.7%). Co-infection by ZIKV and CHIKV was documented in one case. Of the 17 ZIKV-positive individuals, 8 showed reactivity for anti-DENV IgM, which suggested recent DENVinfection, cross-reactivity or co-infection. CONCLUSION: Our findings confirm that accurate laboratory testing is of paramount importance for differential diagnosis in areas of simultaneous transmission of different arboviruses with similar clinical presentations.
Authors: Adam J Reed; Ryan P Connelly; Allison Williams; Maithi Tran; Byoung-Shik Shim; Hyeryun Choe; Yulia V Gerasimova Journal: Sens Actuators B Chem Date: 2018-11-29 Impact factor: 7.460
Authors: Mariana Ruiz Silva; José A Aguilar Briseño; Vinit Upasani; Heidi van der Ende-Metselaar; Jolanda M Smit; Izabela A Rodenhuis-Zybert Journal: PLoS Negl Trop Dis Date: 2017-06-23
Authors: Ludmila Lobkowicz; Anna Ramond; Nuria Sanchez Clemente; Ricardo Arraes de Alencar Ximenes; Demócrito de Barros Miranda-Filho; Ulisses Ramos Montarroyos; Celina Maria Turchi Martelli; Thalia Velho Barreto de Araújo; Elizabeth B Brickley Journal: BMJ Glob Health Date: 2020-05
Authors: Tereza Magalhaes; Cynthia Braga; Marli T Cordeiro; Andre L S Oliveira; Priscila M S Castanha; Ana Paula R Maciel; Nathalia M L Amancio; Pollyanne N Gouveia; Valter J Peixoto-da-Silva; Thaciana F L Peixoto; Helena Britto; Priscilla V Lima; Andreza R S Lima; Kerstin D Rosenberger; Thomas Jaenisch; Ernesto T A Marques Journal: PLoS Negl Trop Dis Date: 2017-11-06