Literature DB >> 2747746

Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos.

P A Maroney1, G J Hannon, T W Nilsen.   

Abstract

High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2747746     DOI： 10.1016/0166-6851(89)90214-4

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  2 in total

1.  Transient expression of DNA and RNA in parasitic helminths by using particle bombardment.

Authors:  R E Davis; A Parra; P T LoVerde; E Ribeiro; G Glorioso; S Hodgson
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

2.  Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system.

Authors:  P A Maroney; G J Hannon; T W Nilsen
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.