Literature DB >> 27476992

A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.

Bin Li1, Troy J Comi1, Tong Si1,2, Sage J B Dunham1, Jonathan V Sweedler3.   

Abstract

Matrix-assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct matrix-assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms.
Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Entities:  

Keywords:  MALDI MSI; airbrush; automatic sprayer; biofilms; matrix application

Mesh：

Substances：

Year:  2016        PMID： 27476992      PMCID： PMC5297451          DOI： 10.1002/jms.3827

Source DB:  PubMed          Journal:  J Mass Spectrom        ISSN： 1076-5174            Impact factor:   1.982


  25 in total

1.  Homogeneous matrix deposition on dried agar for MALDI imaging mass spectrometry of microbial cultures.

Authors:  Thomas Hoffmann; Pieter C Dorrestein
Journal:  J Am Soc Mass Spectrom       Date:  2015-08-22       Impact factor: 3.109

2.  Emerging mass spectrometry techniques for the direct analysis of microbial colonies.

Authors:  Jinshu Fang; Pieter C Dorrestein
Journal:  Curr Opin Microbiol       Date:  2014-07-26       Impact factor: 7.934

3.  Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture.

Authors:  Célio Fernando F Angolini; Pedro Henrique Vendramini; Francisca D S Araújo; Welington L Araújo; Rodinei Augusti; Marcos N Eberlin; Luciana Gonzaga de Oliveira
Journal:  Anal Chem       Date:  2015-06-25       Impact factor: 6.986

Review 4.  Primer on agar-based microbial imaging mass spectrometry.

Authors:  Jane Y Yang; Vanessa V Phelan; Ryan Simkovsky; Jeramie D Watrous; Rachelle M Trial; Tinya C Fleming; Roland Wenter; Bradley S Moore; Susan S Golden; Kit Pogliano; Pieter C Dorrestein
Journal:  J Bacteriol       Date:  2012-07-20       Impact factor: 3.490

Review 5.  Imaging mass spectrometry in microbiology.

Authors:  Jeramie D Watrous; Pieter C Dorrestein
Journal:  Nat Rev Microbiol       Date:  2011-08-08       Impact factor: 60.633

6.  Capturing bacterial metabolic exchange using thin film desorption electrospray ionization-imaging mass spectrometry.

Authors:  Jeramie Watrous; Nathan Hendricks; Michael Meehan; Pieter C Dorrestein
Journal:  Anal Chem       Date:  2010-03-01       Impact factor: 6.986

7.  In situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry.

Authors:  Delphine Debois; Kassem Hamze; Vincent Guérineau; Jean-Pierre Le Caër; I Barry Holland; Philippe Lopes; Jamal Ouazzani; Simone J Séror; Alain Brunelle; Olivier Laprévote
Journal:  Proteomics       Date:  2008-09       Impact factor: 3.984

8.  Multimodal chemical imaging of molecular messengers in emerging Pseudomonas aeruginosa bacterial communities.

Authors:  Nameera F Baig; Sage J B Dunham; Nydia Morales-Soto; Joshua D Shrout; Jonathan V Sweedler; Paul W Bohn
Journal:  Analyst       Date:  2015-09-02       Impact factor: 4.616

9.  MALDI-guided SIMS: multiscale imaging of metabolites in bacterial biofilms.

Authors:  Eric J Lanni; Rachel N Masyuko; Callan M Driscoll; Jordan T Aerts; Joshua D Shrout; Paul W Bohn; Jonathan V Sweedler
Journal:  Anal Chem       Date:  2014-08-26       Impact factor: 6.986

10.  Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging.

Authors:  Tong Si; Bin Li; Ke Zhang; Yiran Xu; Huimin Zhao; Jonathan V Sweedler
Journal:  J Proteome Res       Date:  2016-05-11       Impact factor: 4.466
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  9 in total

1.  Quantitative SIMS Imaging of Agar-Based Microbial Communities.

Authors:  Sage J B Dunham; Joanna F Ellis; Nameera F Baig; Nydia Morales-Soto; Tianyuan Cao; Joshua D Shrout; Paul W Bohn; Jonathan V Sweedler
Journal:  Anal Chem       Date:  2018-04-13       Impact factor: 6.986

2.  microMS: A Python Platform for Image-Guided Mass Spectrometry Profiling.

Authors:  Troy J Comi; Elizabeth K Neumann; Thanh D Do; Jonathan V Sweedler
Journal:  J Am Soc Mass Spectrom       Date:  2017-06-07       Impact factor: 3.109

3.  Response Surface Methodology As a New Approach for Finding Optimal MALDI Matrix Spraying Parameters for Mass Spectrometry Imaging.

Authors:  Dušan Veličković; Guanshi Zhang; Dejan Bezbradica; Arunima Bhattacharjee; Ljiljana Paša-Tolić; Kumar Sharma; Theodore Alexandrov; Christopher R Anderton
Journal:  J Am Soc Mass Spectrom       Date:  2020-02-06       Impact factor: 3.109

4.  Spray-Based Application of Matrix to Agar-Based Microbial Samples for Reproducible Sample Adherence in MALDI MSI.

Authors:  Justin N Jens; Daniel J Breiner; Vanessa V Phelan
Journal:  J Am Soc Mass Spectrom       Date:  2022-02-24       Impact factor: 3.262

5.  macroMS: Image-Guided Analysis of Random Objects by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.

Authors:  Kisurb Choe; Pu Xue; Huimin Zhao; Jonathan V Sweedler
Journal:  J Am Soc Mass Spectrom       Date:  2021-04-06       Impact factor: 3.109

6.  Biopolymer Patterning-Directed Secretion in Mucoid and Nonmucoid Strains of Pseudomonas aeruginosa Revealed by Multimodal Chemical Imaging.

Authors:  Jin Jia; Joanna F Ellis; Tianyuan Cao; Kaiyu Fu; Nydia Morales-Soto; Joshua D Shrout; Jonathan V Sweedler; Paul W Bohn
Journal:  ACS Infect Dis       Date:  2021-02-23       Impact factor: 5.084

7.  Mass Spectrometry Imaging of Complex Microbial Communities.

Authors:  Sage J B Dunham; Joseph F Ellis; Bin Li; Jonathan V Sweedler
Journal:  Acc Chem Res       Date:  2016-12-21       Impact factor: 22.384

8.  Identification, Structure and Characterization of Bacillus tequilensis Biofilm with the Use of Electrophoresis and Complementary Approaches.

Authors:  Katarzyna Pauter; Viorica Railean-Plugaru; Michał Złoch; Paweł Pomastowski; Małgorzata Szultka-Młyńska; Bogusław Buszewski
Journal:  J Clin Med       Date:  2022-01-29       Impact factor: 4.241

Review 9.  "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.

Authors:  Daria Janiszewska; Małgorzata Szultka-Młyńska; Paweł Pomastowski; Bogusław Buszewski
Journal:  Int J Mol Sci       Date:  2022-08-24       Impact factor: 6.208
  9 in total

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