Soo Hyun Ahn1, Kasra Khalaj1, Steven L Young2, Bruce A Lessey3, Madhuri Koti1, Chandrakant Tayade4. 1. Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada. 2. Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina. 3. Department of Obstetrics and Gynecology, Greenville Health Systems, Greenville, South Carolina. 4. Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada. Electronic address: tayadec@queensu.ca.
Abstract
OBJECTIVE: To determine if the molecular profiles of endometriotic lesions contain informative measures of inflammation and immune dysfunction that may contribute to better understanding of the interplay between immune dysfunction and inflammation and their contribution to endometriosis pathogenesis. DESIGN: Immune and inflammation transcriptomic analysis with the use of the Nanostring nCounter GX Human Immunology V2 platform (579 human immune and inflammation-related genes and 15 housekeeping genes). SETTING: Academic university and teaching hospital. INTERVENTION(S): None. PATIENT(S): Stage III-IV endometriosis patients with infertility (n = 8) and fertile disease-free control women undergoing tubal ligation (n = 8). Menstrual stage was matched to secretory phase in all participants. MAIN OUTCOME MEASURE(S): Immune and inflammation transcriptomics quantification from ectopic endometriotic lesions and matched eutopic endometrium from patients. Endometria of fertile women served as control subjects. RESULT(S): Our results displayed endometriotic lesions as molecularly distinct entities compared with eutopic endometrium and endometrium of control samples; 396 out of 579 screened immune and inflammation-related genes were significantly different in ectopic tissues compared with control endometrium. Most importantly, eutopic endometrium of the patients displayed a unique molecular profile compared with the control endometrium (91/579 genes were significantly different), particularly of genes involved in regulation of cell apoptosis and decidualization. CONCLUSION(S): We characterize differential expression of immune-inflammation genes in endometriosis patients, and show molecular distinction of eutopic endometrium of patients compared with control fertile women.
OBJECTIVE: To determine if the molecular profiles of endometriotic lesions contain informative measures of inflammation and immune dysfunction that may contribute to better understanding of the interplay between immune dysfunction and inflammation and their contribution to endometriosis pathogenesis. DESIGN: Immune and inflammation transcriptomic analysis with the use of the Nanostring nCounter GX Human Immunology V2 platform (579 human immune and inflammation-related genes and 15 housekeeping genes). SETTING: Academic university and teaching hospital. INTERVENTION(S): None. PATIENT(S): Stage III-IV endometriosispatients with infertility (n = 8) and fertile disease-free control women undergoing tubal ligation (n = 8). Menstrual stage was matched to secretory phase in all participants. MAIN OUTCOME MEASURE(S): Immune and inflammation transcriptomics quantification from ectopic endometriotic lesions and matched eutopic endometrium from patients. Endometria of fertile women served as control subjects. RESULT(S): Our results displayed endometriotic lesions as molecularly distinct entities compared with eutopic endometrium and endometrium of control samples; 396 out of 579 screened immune and inflammation-related genes were significantly different in ectopic tissues compared with control endometrium. Most importantly, eutopic endometrium of the patients displayed a unique molecular profile compared with the control endometrium (91/579 genes were significantly different), particularly of genes involved in regulation of cell apoptosis and decidualization. CONCLUSION(S): We characterize differential expression of immune-inflammation genes in endometriosispatients, and show molecular distinction of eutopic endometrium of patients compared with control fertile women.
Authors: Yu-Hwa Huang; Chen Zhu; Yasuyuki Kondo; Ana C Anderson; Amit Gandhi; Andrew Russell; Stephanie K Dougan; Britt-Sabina Petersen; Espen Melum; Thomas Pertel; Kiera L Clayton; Monika Raab; Qiang Chen; Nicole Beauchemin; Paul J Yazaki; Michal Pyzik; Mario A Ostrowski; Jonathan N Glickman; Christopher E Rudd; Hidde L Ploegh; Andre Franke; Gregory A Petsko; Vijay K Kuchroo; Richard S Blumberg Journal: Nature Date: 2014-10-26 Impact factor: 49.962
Authors: Richard O Burney; Said Talbi; Amy E Hamilton; Kim Chi Vo; Mette Nyegaard; Camran R Nezhat; Bruce A Lessey; Linda C Giudice Journal: Endocrinology Date: 2007-05-17 Impact factor: 4.736
Authors: Kasra Khalaj; Jessica E Miller; Harshavardhan Lingegowda; Asgerally T Fazleabas; Steven L Young; Bruce A Lessey; Madhuri Koti; Chandrakant Tayade Journal: JCI Insight Date: 2019-09-19
Authors: Laura D Almquist; Creighton E Likes; Benjamin Stone; Kaitlin R Brown; Ricardo Savaris; David A Forstein; Paul B Miller; Bruce A Lessey Journal: Fertil Steril Date: 2017-11-07 Impact factor: 7.329
Authors: Jonathan L Hecht; Monika Janikova; Reeham Choudhury; Fong Liu; Giacomo Canesin; Lubica Janovicova; Eva Csizmadia; Elisa M Jorgensen; Katharine M Esselen; Peter Celec; Kenneth D Swanson; Barbara Wegiel Journal: Cancers (Basel) Date: 2022-04-29 Impact factor: 6.575
Authors: Susan F Evans; Yuen H Kwok; Ann Solterbeck; Jiajun Liu; Mark R Hutchinson; M Louise Hull; Paul E Rolan Journal: J Pain Res Date: 2020-03-09 Impact factor: 3.133