| Literature DB >> 27473482 |
Fernando Benavente1, Silvia Medina-Casanellas2, Estela Giménez2, Victoria Sanz-Nebot2.
Abstract
One of the major drawbacks of capillary electrophoresis (CE) and other microscale separation techniques, for the analysis of low abundant peptides and proteins in complex samples, are the poor concentration limits of detection. Several strategies have been developed to improve CE sensitivity. Here, we describe an on-line solid-phase extraction capillary electrophoresis mass spectrometry method with a commercial C18 sorbent for clean-up and preconcentration of neuropeptides from highly diluted biological samples.Keywords: Analyte concentrator; Capillary electrophoresis; Clean-up; In-line; Mass spectrometry; Microcartridge; On-line; Peptides; Preconcentration; Sensitivity; Solid phase extraction
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Year: 2016 PMID： 27473482 DOI： 10.1007/978-1-4939-4014-1_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745