| Literature DB >> 27473255 |
Awatef El Moussi1,2,3, Michael M Thomson4, Elena Delgado4, María Teresa Cuevas4, Majda Nasr1, Salma Abid1,2, Mohamed Ali Ben Hadj Kacem1,2, Hanene Benaissa Tiouiri5, Amel Letaief6, Mohamed Chakroun7, Mounir Ben Jemaa8, Hayet Hamdouni3,9, Rafla Tej Dellagi9, Khaled Kheireddine3,10, Ilhem Boutiba1,2, Lucía Pérez-Álvarez4, Amine Slim1,2.
Abstract
In this study, the genetic diversity of HIV-1 in Tunisia was analyzed. For this, 193 samples were collected in different regions of Tunisia between 2012 and 2015. A protease and reverse transcriptase fragment were amplified and sequenced. Phylogenetic analyses were performed through maximum likelihood and recombination was analyzed by bootscanning. Six HIV-1 subtypes (B, A1, G, D, C, and F2), 5 circulating recombinant forms (CRF02_AG, CRF25_cpx, CRF43_02G, CRF06_cpx, and CRF19_cpx), and 11 unique recombinant forms were identified. Subtype B (46.4%) and CRF02_AG (39.4%) were the predominant genetic forms. A group of 44 CRF02_AG sequences formed a distinct Tunisian cluster, which also included four viruses from western Europe. Nine viruses were closely related to isolates collected in other African or in European countries. In conclusion, a high HIV-1 genetic diversity is observed in Tunisia and the local spread of CRF02_AG is first documented in this country.Entities:
Keywords: HIV-1; Tunisia; genetic diversity; recombinant forms; unique recombinant forms
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Year: 2016 PMID： 27473255 DOI： 10.1089/AID.2016.0164
Source DB: PubMed Journal: AIDS Res Hum Retroviruses ISSN： 0889-2229 Impact factor: 2.205