Yanxia Lu1, Anand Kumar Andiappan2, Bernett Lee2, Roger Ho3, Tow Keang Lim4, Win Sen Kuan5, Daniel Yam Thiam Goh6, Malcolm Mahadevan5, Tiong Beng Sim5, De Yun Wang7, Hugo P S Van Bever6, Olaf Rotzschke2, Anis Larbi2, Tze Pin Ng8. 1. Department of Clinical Psychology and Psychiatry/School of Public Health, Zhejiang University College of Medicine, Hangzhou, China. 2. Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore. 3. Department of Psychological Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. 4. Division of Respiratory and Critical Care Medicine, Department of Medicine, National University Hospital System, Singapore. 5. Emergency Medicine Department, National University Health System, Singapore. 6. Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. 7. Department of Otolaryngology, National University of Singapore, Singapore. 8. Department of Psychological Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. Electronic address: pcmngtp@nus.edu.sg.
Abstract
OBJECTIVE: Neuropeptide Y, a widely circulating neurotransmitter, plays a pivotal role in energy balance, immunomodulation and asthma, and several NPY polymorphisms are promising genetic risk factors for asthma and obesity. We explored the associations of candidate NPY gene polymorphisms with prevalent asthma and its relationship with obesity in young adult asthma patients free of other chronic medical morbidity. METHODS: Five common gene variants of NPY (rs16147 (-399T/C), rs17149106 (-602G/T), rs16140 (+1000C/G), rs5573 (+1201A/G), rs5574 (+5327C/T)) previously validated to account for most of the NPY expression in vitro and in vivo were investigated in 126 physician-diagnosed asthma patients without other chronic medical morbidity and 182 healthy controls (21-35years). Plasma levels of NPY, adiponectin, and CRP were determined using ELISA, and IL-6 was measured by Luminex in a subgroup of 70 patients and 69 age- and sex-matched healthy controls. RESULTS: In logistic regression models controlling for gender and obesity, the CT genotype of rs5574 (OR=0.54, 95%CI: 0.30-0.89) and the GT genotype of rs17149106 (OR=5.58, 95%CI: 1.09-28.54) were significantly associated with asthma. No significant interaction between NPY SNP polymorphisms and obesity were detected. Plasma NPY level was correlated with adiponectin levels (p<0.05). Compared with the healthy controls, patients with asthma had higher BMI (p<0.001), adiponectin (p<0.05), IL-6 (p=0.001) and CRP (p<0.001), and lower NPY levels (p<0.01). CONCLUSIONS: The CT genotype of rs5574 and the GT genotype of rs17149106 are significantly associated with prevalent asthma.
OBJECTIVE: Neuropeptide Y, a widely circulating neurotransmitter, plays a pivotal role in energy balance, immunomodulation and asthma, and several NPY polymorphisms are promising genetic risk factors for asthma and obesity. We explored the associations of candidate NPY gene polymorphisms with prevalent asthma and its relationship with obesity in young adult asthmapatients free of other chronic medical morbidity. METHODS: Five common gene variants of NPY (rs16147 (-399T/C), rs17149106 (-602G/T), rs16140 (+1000C/G), rs5573 (+1201A/G), rs5574 (+5327C/T)) previously validated to account for most of the NPY expression in vitro and in vivo were investigated in 126 physician-diagnosed asthmapatients without other chronic medical morbidity and 182 healthy controls (21-35years). Plasma levels of NPY, adiponectin, and CRP were determined using ELISA, and IL-6 was measured by Luminex in a subgroup of 70 patients and 69 age- and sex-matched healthy controls. RESULTS: In logistic regression models controlling for gender and obesity, the CT genotype of rs5574 (OR=0.54, 95%CI: 0.30-0.89) and the GT genotype of rs17149106 (OR=5.58, 95%CI: 1.09-28.54) were significantly associated with asthma. No significant interaction between NPY SNP polymorphisms and obesity were detected. Plasma NPY level was correlated with adiponectin levels (p<0.05). Compared with the healthy controls, patients with asthma had higher BMI (p<0.001), adiponectin (p<0.05), IL-6 (p=0.001) and CRP (p<0.001), and lower NPY levels (p<0.01). CONCLUSIONS: The CT genotype of rs5574 and the GT genotype of rs17149106 are significantly associated with prevalent asthma.
Authors: Derek B McMahon; Ryan M Carey; Michael A Kohanski; Nithin D Adappa; James N Palmer; Robert J Lee Journal: Am J Physiol Lung Cell Mol Physiol Date: 2021-03-03 Impact factor: 5.464
Authors: Kheng Siang Ted Ng; Angelia Sia; Maxel K W Ng; Crystal T Y Tan; Hui Yu Chan; Chay Hoon Tan; Iris Rawtaer; Lei Feng; Rathi Mahendran; Anis Larbi; Ee Heok Kua; Roger C M Ho Journal: Int J Environ Res Public Health Date: 2018-08-09 Impact factor: 3.390