Tomasz Zatonski1, Urszula Ciesielska2, Katarzyna Nowinska3, Katarzyna Ratajczak-Wielgomas3, Christopher Kobierzycki3, Bartosz Pula3, Marzenna Podhorska-Okolow3, Tomasz Krecicki1, Piotr Dziegiel4. 1. Department of Otolaryngology, Head and Neck Surgery, Wroclaw Medical University, Wroclaw, Poland. 2. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland urszula.ciesielska@umed.wroc.pl. 3. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland. 4. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland Department of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
Abstract
BACKGROUND: The role of human papillomavirus (HPV) infection as an etiological factor of respiratory tract papillomas has been described in numerous studies, however its role in malignant transformation has not been clearly defined. Depending on their oncogenic potential they have been classified as low- and high-risk HPVs. We analyzed the expression of four cell cycle-related proteins in order to understand the processes leading to malignant transformation. MATERIALS AND METHODS: Fifty-six cases of pharyngeal and laryngeal papillomas were analyzed. Nested multiplex polymerase chain reactions to detect the presence of the HPV types, as well as immunohistochemical reactions were performed for the detection of cell cycle-related proteins p16, p27, p53 and Ki-67. RESULTS: The presence of HPVs 6/11 and 16 was confirmed in 10/56 cases. The expression of all analyzed cell cycle-related proteins was increased in HPV-infected papillomas. CONCLUSION: HPV infection of the upper respiratory tract may influence the expression of cell cycle-related proteins, that could indicate its possible role in the process of malignant transformation. Copyright
BACKGROUND: The role of human papillomavirus (HPV) infection as an etiological factor of respiratory tract papillomas has been described in numerous studies, however its role in malignant transformation has not been clearly defined. Depending on their oncogenic potential they have been classified as low- and high-risk HPVs. We analyzed the expression of four cell cycle-related proteins in order to understand the processes leading to malignant transformation. MATERIALS AND METHODS: Fifty-six cases of pharyngeal and laryngeal papillomas were analyzed. Nested multiplex polymerase chain reactions to detect the presence of the HPV types, as well as immunohistochemical reactions were performed for the detection of cell cycle-related proteins p16, p27, p53 and Ki-67. RESULTS: The presence of HPVs 6/11 and 16 was confirmed in 10/56 cases. The expression of all analyzed cell cycle-related proteins was increased in HPV-infected papillomas. CONCLUSION:HPV infection of the upper respiratory tract may influence the expression of cell cycle-related proteins, that could indicate its possible role in the process of malignant transformation. Copyright