Literature DB >> 27464685

Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay.

Patricia A Jorquera1, Ralph A Tripp2.   

Abstract

One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.

Keywords:  F protein; G protein; HEp-2 cells; Immunostaining; Plaque assay; RSV; VERO cells

Mesh：

Substances：

Year:  2016        PMID： 27464685     DOI： 10.1007/978-1-4939-3687-8_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Verdinexor (KPT-335), a Selective Inhibitor of Nuclear Export, Reduces Respiratory Syncytial Virus Replication In Vitro.

Authors:  Patricia A Jorquera; Cynthia Mathew; Reena Ghildyal; Jennifer Pickens; Colin Williams; Jasmina M Luczo; Sharon Tamir; Ralph A Tripp
Journal:  J Virol       Date:  2019-02-05       Impact factor: 5.103

2.  Respiratory syncytial virus activates Rab5a to suppress IRF1-dependent IFN-λ production, subverting the antiviral defense of airway epithelial cells.

Authors:  Shi Mo; Wei Tang; Jun Xie; Sisi Chen; Luo Ren; Na Zang; Xiaohong Xie; Yu Deng; Leiqiong Gao; Enmei Liu
Journal:  J Virol       Date:  2021-01-27       Impact factor: 6.549

3.  Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus.

Authors:  Sarah Al-Beltagi; Cristian Alexandru Preda; Leah V Goulding; Joe James; Juan Pu; Paul Skinner; Zhimin Jiang; Belinda Lei Wang; Jiayun Yang; Ashley C Banyard; Kenneth H Mellits; Pavel Gershkovich; Christopher J Hayes; Jonathan Nguyen-Van-Tam; Ian H Brown; Jinhua Liu; Kin-Chow Chang
Journal:  Viruses       Date:  2021-02-03       Impact factor: 5.048
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.