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Literature DB >> 27464500

Synthesis and Macrodomain Binding of Mono-ADP-Ribosylated Peptides.

Hans A V Kistemaker¹, Aurelio Pio Nardozza², Herman S Overkleeft¹, Gijs A van der Marel¹, Andreas G Ladurner^3,4,5, Dmitri V Filippov⁶.

Abstract

Mono-ADP-ribosylation is a dynamic posttranslational modification (PTM) with important roles in signaling. Mammalian proteins that recognize or hydrolyze mono-ADP-ribosylated proteins have been described. We report the synthesis of ADP-ribosylated peptides from the proteins histone H2B, RhoA and, HNP-1. An innovative procedure was applied that makes use of pre-phosphorylated amino acid building blocks. Binding assays revealed that the macrodomains of human MacroD2 and TARG1 exhibit distinct specificities for the different ADP-ribosylated peptides, thus showing that the sequence surrounding ADP-ribosylated residues affects the substrate selectivity of macrodomains.

Entities: Chemical Gene Species

Keywords: ADP-ribosylation; peptides; posttranslational modifications; proteins; solid-phase synthesis

Mesh：

Substances：

Year: 2016 PMID： 27464500 DOI： 10.1002/anie.201604058

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

14 in total

1. ADP-ribosylhydrolase activity of Chikungunya virus macrodomain is critical for virus replication and virulence.

Authors: Robert Lyle McPherson; Rachy Abraham; Easwaran Sreekumar; Shao-En Ong; Shang-Jung Cheng; Victoria K Baxter; Hans A V Kistemaker; Dmitri V Filippov; Diane E Griffin; Anthony K L Leung
Journal: Proc Natl Acad Sci U S A Date: 2017-01-31 Impact factor: 11.205

2. ELTA: Enzymatic Labeling of Terminal ADP-Ribose.

Authors: Yoshinari Ando; Elad Elkayam; Robert Lyle McPherson; Morgan Dasovich; Shang-Jung Cheng; Jim Voorneveld; Dmitri V Filippov; Shao-En Ong; Leemor Joshua-Tor; Anthony K L Leung
Journal: Mol Cell Date: 2019-01-31 Impact factor: 17.970

3. New Quantitative Mass Spectrometry Approaches Reveal Different ADP-ribosylation Phases Dependent On the Levels of Oxidative Stress.

Authors: Vera Bilan; Nathalie Selevsek; Hans A V Kistemaker; Jeannette Abplanalp; Roxane Feurer; Dmitri V Filippov; Michael O Hottiger
Journal: Mol Cell Proteomics Date: 2017-03-21 Impact factor: 5.911

4. Molecular Tools for the Study of ADP-Ribosylation: A Unified and Versatile Method to Synthesise Native Mono-ADP-Ribosylated Peptides.

Authors: Jim Voorneveld; Johannes Gregor Matthias Rack; Luke van Gijlswijk; Nico J Meeuwenoord; Qiang Liu; Herman S Overkleeft; Gijsbert A van der Marel; Ivan Ahel; Dmitri V Filippov
Journal: Chemistry Date: 2021-05-06 Impact factor: 5.020

Synthesis and Macrodomain Binding of Mono-ADP-Ribosylated Peptides.

1. ADP-ribosylhydrolase activity of Chikungunya virus macrodomain is critical for virus replication and virulence.

2. ELTA: Enzymatic Labeling of Terminal ADP-Ribose.

3. New Quantitative Mass Spectrometry Approaches Reveal Different ADP-ribosylation Phases Dependent On the Levels of Oxidative Stress.

4. Molecular Tools for the Study of ADP-Ribosylation: A Unified and Versatile Method to Synthesise Native Mono-ADP-Ribosylated Peptides.

5. Proteomic analyses identify ARH3 as a serine mono-ADP-ribosylhydrolase.

Review 6. PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes.

7. ADP-ribosyl-N₃: A Versatile Precursor for Divergent Syntheses of ADP-ribosylated Compounds.

Review 8. (ADP-ribosyl)hydrolases: structure, function, and biology.

9. Both ADP-Ribosyl-Binding and Hydrolase Activities of the Alphavirus nsP3 Macrodomain Affect Neurovirulence in Mice.

Review 10. ADP-ribosylation: new facets of an ancient modification.