Fatima AlZahra'a Alatraktchi1,2,3, Helle Krogh Johansen3,4, Søren Molin2,3, Winnie Edith Svendsen1. 1. Department of Micro- & Nanotechnology, Technical University of Denmark, Kgs-Lyngby, Denmark. 2. Department of Biotechnology & Biomedicine, Technical University of Denmark, Kgs-Lyngby, Denmark. 3. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark. 4. Cystisk Fibrose Klinikken & Klinisk Mikrobiologisk Afdeling - Rigshospitalet, Copenhagen, Denmark.
Abstract
AIM: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. MATERIALS & METHODS: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. RESULTS & CONCLUSION: Pyocyanin was detected at concentrations down to 125 nM in a 50 µM mixture of interfering compounds with a reproducibility of r(2) = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.
AIM: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. MATERIALS & METHODS:Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions. RESULTS & CONCLUSION:Pyocyanin was detected at concentrations down to 125 nM in a 50 µM mixture of interfering compounds with a reproducibility of r(2) = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.
Authors: Marwa M Khalifa; Amal A Elkhawaga; Mona A Hassan; Asmaa M Zahran; Ahmed M Fathalla; Waleed A El-Said; Omnia El-Badawy Journal: Sci Rep Date: 2019-12-04 Impact factor: 4.379