Jian Bo1, Lu Sun2, Wenqing Wang3, Chao Ma1, Honghua Li1, Yu Zhao1. 1. Department of Hematology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China. 2. Departments of Pathology, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100853, China. 3. Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China.
Abstract
AIM OF STUDY: Novel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance of Non-Hodgkin's lymphomas (NHL). So we explored the viability of analytical methods for identifying the rearranged immunoglobulin (Ig) H genes sequence. MATERIALS AND METHODS: Next-generation sequencing (NGS) was used to sequence deoxyribonucleic acid (DNA) extracted directly from the tumor tissues of patients with NHL, and then specific rearranged DNA fragments from plasma was detected by polymerase chain reaction (PCR). RESULTS: By parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 patients with NHL. The circulating rearranged DNA fragments had been identified in the plasma of one patient. CONCLUSION: IgCap may be the favorable diagnostic method for patients with NHL in clinical.
AIM OF STUDY: Novel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance of Non-Hodgkin's lymphomas (NHL). So we explored the viability of analytical methods for identifying the rearranged immunoglobulin (Ig) H genes sequence. MATERIALS AND METHODS: Next-generation sequencing (NGS) was used to sequence deoxyribonucleic acid (DNA) extracted directly from the tumor tissues of patients with NHL, and then specific rearranged DNA fragments from plasma was detected by polymerase chain reaction (PCR). RESULTS: By parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 patients with NHL. The circulating rearranged DNA fragments had been identified in the plasma of one patient. CONCLUSION: IgCap may be the favorable diagnostic method for patients with NHL in clinical.