Hyo Won Jung1,2, Kyung-Ho Kim3, Yong-Ki Park4,5. 1. Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 740-814, Republic of Korea. tenzing2@hanmail.net. 2. Korean Medicine Research and Development Center, Dongguk University, Gyeongju, 740-814, Republic of Korea. tenzing2@hanmail.net. 3. Department of Acupuncture, College of Korean Medicine, Dongguk University, Gyeongju, 740-814, Republic of Korea. 4. Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 740-814, Republic of Korea. 5. Korean Medicine Research and Development Center, Dongguk University, Gyeongju, 740-814, Republic of Korea.
Abstract
OBJECTIVE: To investigate whether the dried root of Phellodendron amurense Ruprecht (Phellodendri cortex; PC) extract improves arthritic symptoms through anti-inflammatory and immune-modulatory effects in collagen-induced arthritis in mice. METHODS: Rheumatoid arthritis (RA) was induced in male DBA/1 mice by immunization with type II collagen (ColII). CIA mice were divided into 5 groups (n=10 per a group) with normal, CIA control, PC extract (50 mg/kg and 100 mg/kg)-treated, and meloxicam (50 mg/kg)-treated as the reference drug. The PC extract or meloxicam were administered orally in CIA mice once a day for 14 days after arthritis induction. Arthritic score, levels of anti-ColII IgG2a antibody, prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α, and interleukin (IL)-17 in the sera of CIA mice were measured. Histopathological changes in the ankle joints of CIA mice were also analyzed by staining with hematoxylin and eosin (H and E), safranin-O and immunohistochemistry using anti-TNF-α and anti-IL-17 antibodies. RESULTS: The arthritic score was increased in CIA mice in a time-dependent manner, as were the serum levels of anti-ColII IgG2a antibody, PGE2, TNF-α, and IL-17. However, the oral administration of PC extract at 50 and 100 mg/kg in CIA mice significantly decreased the arthritic scores, and the serum levels of anti-ColII IgG2a, PGE2, TNF-α, and IL-17 compared with those in the CIA group (P<0.05 or P<0.01). Furthermore, histopathological improvement of the joint architecture in CIA mice was observed after administration of PC extract. PC extract also significantly inhibited the expression of TNF-α and IL-17 in the joints of CIA mice by suppressing the expression of their mRNA and proteins. CONCLUSION: PC extract may improve the pathological progression of RA through the inhibition of joint destruction by synovial inflammation and immune-stimulation, therefore, it would be a potential anti-arthritic agent in RA.
OBJECTIVE: To investigate whether the dried root of Phellodendron amurense Ruprecht (Phellodendri cortex; PC) extract improves arthritic symptoms through anti-inflammatory and immune-modulatory effects in collagen-induced arthritis in mice. METHODS:Rheumatoid arthritis (RA) was induced in male DBA/1 mice by immunization with type II collagen (ColII). CIAmice were divided into 5 groups (n=10 per a group) with normal, CIA control, PC extract (50 mg/kg and 100 mg/kg)-treated, and meloxicam (50 mg/kg)-treated as the reference drug. The PC extract or meloxicam were administered orally in CIAmice once a day for 14 days after arthritis induction. Arthritic score, levels of anti-ColII IgG2a antibody, prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-α, and interleukin (IL)-17 in the sera of CIAmice were measured. Histopathological changes in the ankle joints of CIAmice were also analyzed by staining with hematoxylin and eosin (H and E), safranin-O and immunohistochemistry using anti-TNF-α and anti-IL-17 antibodies. RESULTS: The arthritic score was increased in CIAmice in a time-dependent manner, as were the serum levels of anti-ColII IgG2a antibody, PGE2, TNF-α, and IL-17. However, the oral administration of PC extract at 50 and 100 mg/kg in CIAmice significantly decreased the arthritic scores, and the serum levels of anti-ColII IgG2a, PGE2, TNF-α, and IL-17 compared with those in the CIA group (P<0.05 or P<0.01). Furthermore, histopathological improvement of the joint architecture in CIAmice was observed after administration of PC extract. PC extract also significantly inhibited the expression of TNF-α and IL-17 in the joints of CIAmice by suppressing the expression of their mRNA and proteins. CONCLUSION:PC extract may improve the pathological progression of RA through the inhibition of joint destruction by synovial inflammation and immune-stimulation, therefore, it would be a potential anti-arthritic agent in RA.
Authors: J I Park; J K Shim; J W Do; S Y Kim; E K Seo; H J Kwon; T K Lee; J K Kim; D Y Choi; C H Kim Journal: Glycoconj J Date: 1999-03 Impact factor: 2.916
Authors: Ľudmila Balážová; Slavomír Kurhajec; Martin Kello; Zdenka Bedlovičová; Martina Zigová; Eva Petrovová; Katarína Beňová; Ján Mojžiš; Jarmila Eftimová Journal: Life (Basel) Date: 2022-05-21