Literature DB >> 27460427

A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common gastroenteritis pathogens in Botswana.

Margaret Mokomane1, Ishmael Kasvosve2, Simani Gaseitsiwe3, Andrew P Steenhoff4, Jeffrey M Pernica5, Kwana Lechiile6, Kathy Luinstra7, Marek Smieja5, David M Goldfarb8.   

Abstract

We compared the performance of flocked and matched traditional rectal swabs collected from 236 children admitted with gastroenteritis in Botswana. All samples were tested using real time multiplex-PCR assays for nine enteric pathogens. There was a 20% higher detection of Shigella from flocked swabs, but most other pathogens had similar detection rates.
Copyright © 2016 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Flocked swabs; Gastroenteritis; McNemar exact test; Multiplex PCR; Shigella; Threshold cycle

Mesh：

Year:  2016        PMID： 27460427      PMCID： PMC5028275          DOI： 10.1016/j.diagmicrobio.2016.07.007

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  18 in total

1.  Comparison of flocked and rayon swabs for collection of respiratory epithelial cells from uninfected volunteers and symptomatic patients.

Authors:  Peter Daley; Santina Castriciano; Max Chernesky; Marek Smieja
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

2.  Multiplex real-time PCR for exhaustive detection of diarrhoeagenic Escherichia coli.

Authors:  A Hidaka; T Hokyo; K Arikawa; S Fujihara; J Ogasawara; A Hase; Y Hara-Kudo; Y Nishikawa
Journal:  J Appl Microbiol       Date:  2009-02       Impact factor: 3.772

3.  Post-coital vaginal sampling with nylon flocked swabs improves DNA typing.

Authors:  Corina C G Benschop; Danielle C Wiebosch; Ate D Kloosterman; Titia Sijen
Journal:  Forensic Sci Int Genet       Date:  2009-08-14       Impact factor: 4.882

4.  Evaluation of Copan FLOQSwab for the molecular detection of Chlamydia trachomatis by Abbott RealTime CT PCR.

Authors:  L Coorevits; C Vanscheeuwijck; A Traen; L Bingé; I Ryckaert; E Padalko
Journal:  Acta Clin Belg       Date:  2015-06-24       Impact factor: 1.264

5.  Global burden of Shigella infections: implications for vaccine development and implementation of control strategies.

Authors:  K L Kotloff; J P Winickoff; B Ivanoff; J D Clemens; D L Swerdlow; P J Sansonetti; G K Adak; M M Levine
Journal:  Bull World Health Organ       Date:  1999       Impact factor: 9.408

6.  Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection.

Authors:  S Stacy-Phipps; J J Mecca; J B Weiss
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

7.  Hospitalization and mortality among primarily nonbreastfed children during a large outbreak of diarrhea and malnutrition in Botswana, 2006.

Authors:  Tracy L Creek; Andrea Kim; Lydia Lu; Anna Bowen; Japhter Masunge; Wences Arvelo; Molly Smit; Ondrej Mach; Keitumetse Legwaila; Catherine Motswere; Laurel Zaks; Thomas Finkbeiner; Laura Povinelli; Maruping Maruping; Gibson Ngwaru; Goitebetswe Tebele; Cheryl Bopp; Nancy Puhr; Stephanie P Johnston; Alexandre J Dasilva; Caryn Bern; R S Beard; Margarett K Davis
Journal:  J Acquir Immune Defic Syndr       Date:  2010-01       Impact factor: 3.731

8.  Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage.

Authors:  Rebecca A Guy; Pierre Payment; Ulrich J Krull; Paul A Horgen
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

9.  Risk factors for death among children less than 5 years old hospitalized with diarrhea in rural western Kenya, 2005-2007: a cohort study.

Authors:  Ciara E O'Reilly; Peter Jaron; Benjamin Ochieng; Amek Nyaguara; Jacqueline E Tate; Michele B Parsons; Cheryl A Bopp; Kara A Williams; Jan Vinjé; Elizabeth Blanton; Kathleen A Wannemuehler; John Vulule; Kayla F Laserson; Robert F Breiman; Daniel R Feikin; Marc-Alain Widdowson; Eric Mintz
Journal:  PLoS Med       Date:  2012-07-03       Impact factor: 11.069

10.  Enterotoxigenic Escherichia coli is detectable in water samples from an endemic area by real-time PCR.

Authors:  A Lothigius; A Janzon; Y Begum; A Sjöling; F Qadri; A-M Svennerholm; I Bölin
Journal:  J Appl Microbiol       Date:  2007-11-01       Impact factor: 3.772
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  2 in total

1.  Optimising the management of childhood acute diarrhoeal disease using a rapid test-and- treat strategy and/or Lactobacillus reuteri DSM 17938: a multicentre, randomised, controlled, factorial trial in Botswana.

Authors:  Jeffrey M Pernica; Tonya Arscott-Mills; Andrew P Steenhoff; Margaret Mokomane; Banno Moorad; Mbabi Bapabi; Kwana Lechiile; Oarabile Mangwegape; Boswa Batisani; Norah Mawoko; Charles Muthoga; Thuvaraha Vanniyasingam; Joycelyne Ewusie; Amy Lowe; Janice M Bonsu; Alemayehu M Gezmu; Marek Smieja; Loeto Mazhani; Ketil Stordal; Lehana Thabane; Matthew S Kelly; David M Goldfarb
Journal:  BMJ Glob Health       Date:  2022-04

2.  Rapid enteric testing to permit targeted antimicrobial therapy, with and without Lactobacillus reuteri probiotics, for paediatric acute diarrhoeal disease in Botswana: A pilot, randomized, factorial, controlled trial.

Authors:  Jeffrey M Pernica; Andrew P Steenhoff; Margaret Mokomane; Banno Moorad; Kwana Lechiile; Marek Smieja; Loeto Mazhani; Ji Cheng; Matthew S Kelly; Mark Loeb; Ketil Stordal; David M Goldfarb
Journal:  PLoS One       Date:  2017-10-09       Impact factor: 3.240
  2 in total

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