| Literature DB >> 27460234 |
Ivan A Vorobjev1,2, Natasha S Barteneva3,4.
Abstract
This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities' distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.Keywords: Autofluorescence; Automated image analysis; Flow cytometry; Imaging flow cytometry; Quantitative morphology; Spectral compensation
Mesh：
Year: 2016 PMID： 27460234 DOI： 10.1007/978-1-4939-3302-0_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745