P Ratajczak1, K Kus1, K Gołembiowska2, K Noworyta-Sokołowska2, A Woźniak3, T Zaprutko1, E Nowakowska4. 1. Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Dabrowskiego 79, 60-529 Poznan, Poland. 2. Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Cracow, Poland. 3. Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland. 4. Department of Pharmacoeconomics and Social Pharmacy, Poznan University of Medical Sciences, Dabrowskiego 79, 60-529 Poznan, Poland. Electronic address: elapharm@ump.edu.pl.
Abstract
OBJECTIVES: The study aims to verify whether alterations in the level of neurotransmitters have occurred in prenatally stressed rats (animal model of schizophrenia), and whether aripiprazole (ARI) and olanzapine (OLA) modify this level. METHODS: The effects of ARI (1.5mg/kg) and OLA (0.5mg/kg) were studied by means of microdialysis in freely moving rats (observation time 120min). The level of neurotransmitters (DA, 5-HT, NA) and their metabolites (DOPAC, HVA, 5-HIAA) was analyzed by HPLC with coulochemical detection. RESULTS: Obtained results indicate that after a single administration of ARI and OLA in the prenatally stressed rats the increase of DA, DOPAC, and 5-HT was observed. In turn ARI administration increase the level of HVA and 5-HIAA and also decrease the level of NA. After OLA administration the level of NA and HVA increased and no significant change in 5-HIAA was observed. CONCLUSION: Alterations observed as a result of ARI and OLA administration may be pivotal in identifying animal models of mental disorders and in the analysis of neuroleptics effectiveness.
OBJECTIVES: The study aims to verify whether alterations in the level of neurotransmitters have occurred in prenatally stressed rats (animal model of schizophrenia), and whether aripiprazole (ARI) and olanzapine (OLA) modify this level. METHODS: The effects of ARI (1.5mg/kg) and OLA (0.5mg/kg) were studied by means of microdialysis in freely moving rats (observation time 120min). The level of neurotransmitters (DA, 5-HT, NA) and their metabolites (DOPAC, HVA, 5-HIAA) was analyzed by HPLC with coulochemical detection. RESULTS: Obtained results indicate that after a single administration of ARI and OLA in the prenatally stressed rats the increase of DA, DOPAC, and 5-HT was observed. In turn ARI administration increase the level of HVA and 5-HIAA and also decrease the level of NA. After OLA administration the level of NA and HVA increased and no significant change in 5-HIAA was observed. CONCLUSION: Alterations observed as a result of ARI and OLA administration may be pivotal in identifying animal models of mental disorders and in the analysis of neuroleptics effectiveness.
Authors: Despina E Ganella; Liubov Lee-Kardashyan; Sophia J Luikinga; Danny L D Nguyen; Heather B Madsen; Isabel C Zbukvic; Russell Coulthard; Andrew J Lawrence; Jee Hyun Kim Journal: Front Behav Neurosci Date: 2017-05-09 Impact factor: 3.558
Authors: Anya Savransky; Joshua Chiappelli; Feven Fisseha; Krista M Wisner; Du Xiaoming; S Milad Mirmomen; Aaron D Jones; Bhim M Adhikari; Heather A Bruce; Laura M Rowland; L Elliot Hong Journal: Transl Psychiatry Date: 2018-11-12 Impact factor: 6.222