Teresa Kilian1, Florian Fidler2, Annika Kasten3, Sarah Nietzer4, Veronika Landgraf4, Katrin Weiß4, Heike Walles1,4, Fritz Westphal5, Stephan Hackenberg6, Cordula Grüttner5, Maria Steinke1,4. 1. Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center "Regenerative Therapies for Oncology & Musculoskeletal Diseases" - Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany. 2. Research Center Magnetic-Resonance-Bavaria, Am Hubland, 97074 Würzburg, Germany. 3. Department of Oral & Maxillofacial Surgery, Facial Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany. 4. Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany. 5. Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Straße 4, 18119 Rostock, Germany. 6. Department of Oto-Rhino-Laryngology, Plastic, Aesthetic & Reconstructive Head & Neck Surgery, University Hospital Würzburg, Josef-Schneider-Straße 11, 97080 Würzburg, Germany.
Abstract
AIM: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. MATERIALS & METHODS: We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively. RESULTS: M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture. CONCLUSION: M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.
AIM: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. MATERIALS & METHODS: We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively. RESULTS: M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture. CONCLUSION: M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.
Entities:
Keywords:
metal nanoparticles; regenerative medicine; stem cells
Authors: Katrin Radeloff; Andreas Radeloff; Mario Ramos Tirado; Agmal Scherzad; Rudolf Hagen; Norbert H Kleinsasser; Stephan Hackenberg Journal: Nanomaterials (Basel) Date: 2020-04-13 Impact factor: 5.076