| Literature DB >> 27453899 |
Abstract
Cell membranes from Caulobacter can be isolated and separated into inner and outer membranes according their characteristic buoyant densities on a sucrose gradient. Fractionation can be used to determine the localisation of uncharacterised proteins and to enrich protein complexes present in either of these membranes for biochemical analysis such blue-native PAGE and immunoprecipitation.Entities:
Year: 2012 PMID: 27453899 PMCID: PMC4957949 DOI: 10.21769/bioprotoc.276
Source DB: PubMed Journal: Bio Protoc ISSN: 2331-8325