Histological location of myxosporean plasmodia in fish tissue with Luna's method.

In the present study, a staining technique, Luna's method, has been used for detection and tissue location of myxosporean spores/plasmodia in histological sections of gills and liver. The Luna's staining was performed on the sections of the tissue fixed in Bouin's, embedded in paraffin wax. The mature plasmodia were stained in bright red color and pansporoblasts and gill tissue stained in blue. This staining helped to detect and locate even a single myxospore of less than 15 μm in size located within the tissues of the host.