Guillerm Ignacio Perez-Perez1, Thinh Nguyen Van2, Duong Thu Huong3, Gao Zhan4, Do Nguyet Anh5, Nguyen Thi Nguyet3, Loan Ta Thi3, Nguyen Van Thinh3, Nguyen Thi Hong-Hanh6. 1. Department of Medicine, NYU Langone Medical Center, New York, NY; Department Microbiology, NYU Langone Medical Center, New York, NY. Electronic address: Perezg02@med.nyu.edu. 2. Buu Dien Hospital, Hanoi, Vietnam. 3. Institute of Biotechnology, Vietnam Academy of Sciences and Technology. 4. Department of Medicine, NYU Langone Medical Center, New York, NY. 5. E Hospital, Hanoi, Vietnam. 6. Institute of Biotechnology, Vietnam Academy of Sciences and Technology; Research and Development Center for Biotechnology CBT, VUSTA.
Abstract
BACKGROUND AND AIM: Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients. METHODS: We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR. RESULTS: H. pylori were recovered under anaerobic and micro-aerobic conditions from gastric biopsies in 16 patients. Anaerobic conditions yielded significantly higher H. pylori recovery rates than micro-aerobic conditions (81.3% vs. 31.3%, P= 0.01). H. pylori isolates were characterized by PCR for specific virulence markers and the genotypes were similar to those previously described in this region of the world. CONCLUSIONS: H. pylori can be isolated under anaerobic conditions. These findings may provide new insight into the physiology of this human pathogen and help to identify the route of H. pylori transmission.
BACKGROUND AND AIM: Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pyloriinfectedpatients. METHODS: We enrolled 27 patients with bleeding (erosive) gastritis (mean age 36.3 years, 55.6% male) from Hanoi, Vietnam. H. pylori status was confirmed by qPCR. RESULTS:H. pylori were recovered under anaerobic and micro-aerobic conditions from gastric biopsies in 16 patients. Anaerobic conditions yielded significantly higher H. pylori recovery rates than micro-aerobic conditions (81.3% vs. 31.3%, P= 0.01). H. pylori isolates were characterized by PCR for specific virulence markers and the genotypes were similar to those previously described in this region of the world. CONCLUSIONS:H. pylori can be isolated under anaerobic conditions. These findings may provide new insight into the physiology of this human pathogen and help to identify the route of H. pylori transmission.
Authors: Stephanie Bury-Moné; Nadeem O Kaakoush; Corinne Asencio; Francis Mégraud; Marie Thibonnier; Hilde De Reuse; George L Mendz Journal: Helicobacter Date: 2006-08 Impact factor: 5.753
Authors: Wagih A El-Shouny; Sameh S Ali; Hegazy M Hegazy; Manar K Abd Elnabi; Asmaa Ali; Jianzhong Sun Journal: J Tradit Complement Med Date: 2019-05-15