K Vocetkova1,2,3,4, M Buzgo5,6,7, V Sovkova5,6, D Bezdekova5,6, P Kneppo8, E Amler5,6,7. 1. Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic. karolina_vocetkova@labdemo.cz. 2. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic. karolina_vocetkova@labdemo.cz. 3. University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic. karolina_vocetkova@labdemo.cz. 4. Faculty of Biomedical Engineering, Czech Technical University in Prague, 272 01, Kladno 2, Czech Republic. karolina_vocetkova@labdemo.cz. 5. Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic. 6. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic. 7. University Center for Energy Efficient Buildings, Czech Technical University in Prague, 273 43, Bustehrad, Czech Republic. 8. Faculty of Biomedical Engineering, Czech Technical University in Prague, 272 01, Kladno 2, Czech Republic.
Abstract
OBJECTIVES: Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. METHODS: In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. RESULTS: The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. CONCLUSIONS: Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.
OBJECTIVES: Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. METHODS: In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated. RESULTS: The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells. CONCLUSIONS: Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.
Authors: Petr Knotek; Miloslav Pouzar; Matej Buzgo; Barbora Krizkova; Milan Vlcek; Andrea Mickova; Martin Plencner; Jakub Navesnik; Evzen Amler; Petr Belina Journal: Mater Sci Eng C Mater Biol Appl Date: 2012-04-18 Impact factor: 7.328
Authors: B East; J Woleský; R Divín; M Otáhal; K Vocetková; V Sovková; V H Blahnová; M Koblížek; P Kubový; A Nečasová; A Staffa; A Ch de Beaux; J Lorenzová; E Amler Journal: Hernia Date: 2022-04-04 Impact factor: 4.739
Authors: Mohammad Mahdian-Dehkordi; Farshid Sarrafzadeh-Rezaei; Mazdak Razi; Mehdi Mahmoudian Journal: Vet Res Forum Date: 2021-03-15 Impact factor: 1.054
Authors: Karolina Vocetkova; Vera Sovkova; Matej Buzgo; Vera Lukasova; Radek Divin; Michala Rampichova; Pavel Blazek; Tomas Zikmund; Jozef Kaiser; Zdenek Karpisek; Evzen Amler; Eva Filova Journal: Nanomaterials (Basel) Date: 2020-09-10 Impact factor: 5.076