Emilie Dussossoy1, Florence Bichon2, Emilie Bony3, Karine Portet4, Pierre Brat5, Fabrice Vaillant6, Alain Michel7, Patrick Poucheret8. 1. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: emilie.dussossoy@etu.umontpellier.fr. 2. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: florence.bichon@umontpellier.fr. 3. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: emilie.bony@etu.umontpellier.fr. 4. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: karine.portet@umontpellier.fr. 5. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France. Electronic address: pierre.brat@cirad.fr. 6. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France. Electronic address: fabrice.vaillant@cirad.fr. 7. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: alain.michel@univ-montp1.fr. 8. Laboratoire de pharmacologie et physiopathologie expérimentales, UMR Qualisud, Faculté de pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France. Electronic address: patrick.poucheret@umontpellier.fr.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Morinda citrifolia L. (Noni) is a medicinal plant used in Polynesia for many properties such as anti-inflammatory, anti-diabetic and antineoplastic effects. Recent studies showed that noni juice have anti-oxidant and acute anti-inflammatory activities likely due to polyphenols, iridoids and vitamin C content. The present study was undertaken to evaluate chronic anti-inflammatory and spasmolytic effects of noni juice. MATERIALS AND METHODS: Therefore, we evaluated the effect of oral or intraperitoneal administrations of noni juice in vivo on the lung inflammation in ovalbumin (OVA) sensitized Brown Norway rat (with prednisolone 10mg/kg intraperitoneously as reference compound) and the ex vivo effect of noni juice on BaCl2 (calcium signal) or methacholine (cholinergic signal) induced spasms in jejunum segments. RESULTS: We found that noni juice (intraperitoneously 2.17mL/kg and orally 4.55mL/kg) reduced the inflammation in OVA-sensitized Brown Norway rat with regard to the decreased number of inflammatory cells in lung (macrophages minus 20-26%, lymphocytes minus 58-34%, eosinophils minus 53-30%, neutrophils minus 70-28% respectively). Noni juice demonstrated a dose-dependent NO scavenging effect up to 8.1nmol of nitrites for 50µL of noni juice. In addition noni juice inhibited (up to 90%) calcium and cholinergic induced spasms on the jejunum segments model with a rightward shift of the concentration response curve. CONCLUSION: We describe for the first time that noni juice demonstrate (1) a chronic anti-inflammatory activity on sensitized lungs along with (2) a spasmolytic effect integrating a calcium channel blocker activity component.
ETHNOPHARMACOLOGICAL RELEVANCE: Morinda citrifolia L. (Noni) is a medicinal plant used in Polynesia for many properties such as anti-inflammatory, anti-diabetic and antineoplastic effects. Recent studies showed that noni juice have anti-oxidant and acute anti-inflammatory activities likely due to polyphenols, iridoids and vitamin C content. The present study was undertaken to evaluate chronic anti-inflammatory and spasmolytic effects of noni juice. MATERIALS AND METHODS: Therefore, we evaluated the effect of oral or intraperitoneal administrations of noni juice in vivo on the lung inflammation in ovalbumin (OVA) sensitized Brown Norway rat (with prednisolone 10mg/kg intraperitoneously as reference compound) and the ex vivo effect of noni juice on BaCl2 (calcium signal) or methacholine (cholinergic signal) induced spasms in jejunum segments. RESULTS: We found that noni juice (intraperitoneously 2.17mL/kg and orally 4.55mL/kg) reduced the inflammation in OVA-sensitized Brown Norway rat with regard to the decreased number of inflammatory cells in lung (macrophages minus 20-26%, lymphocytes minus 58-34%, eosinophils minus 53-30%, neutrophils minus 70-28% respectively). Noni juice demonstrated a dose-dependent NO scavenging effect up to 8.1nmol of nitrites for 50µL of noni juice. In addition noni juice inhibited (up to 90%) calcium and cholinergic induced spasms on the jejunum segments model with a rightward shift of the concentration response curve. CONCLUSION: We describe for the first time that noni juice demonstrate (1) a chronic anti-inflammatory activity on sensitized lungs along with (2) a spasmolytic effect integrating a calcium channel blocker activity component.