Khairunnisa A Ghaffar , Nirmal Marasini , Ashwini K Giddam , Michael R Batzloff , Michael F Good , Mariusz Skwarczynski 1 , Istvan Toth 2 Show Affiliations »
Abstract
BACKGROUND: Group A streptococcus (GAS) is an exclusively human pathogenic bacteria. A delay in treatment of GAS infection often lead to severe diseases such as rheumatic heart disease which attributes to hundreds of thousands deaths annually. For the past few decades, the quest for a commercial GAS vaccine has been futile. Currently one of the most investigated strategies to develop vaccine against GAS includes the use of conserved epitopes from major virulent factor of GAS, M-protein. METHODS: In this study, cationic liposomes of various sizes (70 nm to 1000 nm) were prepared with dimethyldioctadecylammonium bromide (DDAB) encapsulating lipopeptide bearing M-protein derived B-cell epitope (J14). RESULTS: Smaller liposomes induced higher antibody titres, though the differences between groups were not statistically significant. CONCLUSION: Nonetheless, all mice which were immunized with liposome-lipopeptide delivery system elicited high levels of systemic (IgG) and mucosal antibodies (IgA), which were discernably higher than those induced with the help of commercial adjuvant (cholera toxin B subunit). Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
BACKGROUND: Group A streptococcus (GAS ) is an exclusively human pathogenic bacteria. A delay in treatment of GAS infection often lead to severe diseases such as rheumatic heart disease which attributes to hundreds of thousands deaths annually. For the past few decades, the quest for a commercial GAS vaccine has been futile. Currently one of the most investigated strategies to develop vaccine against GAS includes the use of conserved epitopes from major virulent factor of GAS , M-protein. METHODS: In this study, cationic liposomes of various sizes (70 nm to 1000 nm) were prepared with dimethyldioctadecylammonium bromide (DDAB ) encapsulating lipopeptide bearing M-protein derived B-cell epitope (J14). RESULTS: Smaller liposomes induced higher antibody titres, though the differences between groups were not statistically significant. CONCLUSION: Nonetheless, all mice which were immunized with liposome-lipopeptide delivery system elicited high levels of systemic (IgG) and mucosal antibodies (IgA), which were discernably higher than those induced with the help of commercial adjuvant (cholera toxin B subunit). Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
Entities: Chemical
Disease
Species
Keywords:
Peptide-based subunit vaccine; Streptococcus pyogenes; liposomes; mucosal immunity; self-adjuvanting.
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Year: 2016
PMID： 27449794 DOI： 10.2174/1573406412666160720093138
Source DB: PubMed Journal: Med Chem ISSN： 1573-4064 Impact factor: 2.745