Literature DB >> 27447465

Substitution of a conserved catalytic dyad into 2-KPCC causes loss of carboxylation activity.

Gregory A Prussia1, George H Gauss1, Florence Mus1, Leah Conner1, Jennifer L DuBois1, John W Peters1.   

Abstract

The characteristic His-Glu catalytic dyad of the disulfide oxidoreductase (DSOR) family of enzymes is replaced in 2-ketopropyl coenzyme M oxidoreductase/carboxylase (2-KPCC) by the residues Phe-His. 2-KPCC is the only known carboxylating member of the DSOR family and has replaced this dyad potentially to eliminate proton-donating groups at a key position in the active site. Substitution of the Phe-His by the canonical residues results in production of higher relative concentrations of acetone versus the natural product acetoacetate. The results indicate that these differences in 2-KPCC are key in discriminating between carbon dioxide and protons as attacking electrophiles.
© 2016 Federation of European Biochemical Societies.

Entities:  

Keywords:  carboxylation; catalytic dyad; protonation

Mesh:

Substances:

Year:  2016        PMID: 27447465     DOI: 10.1002/1873-3468.12325

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  4 in total

1.  The reactive form of a C-S bond-cleaving, CO2-fixing flavoenzyme.

Authors:  Bennett R Streit; Jenna R Mattice; Gregory A Prussia; John W Peters; Jennifer L DuBois
Journal:  J Biol Chem       Date:  2019-01-29       Impact factor: 5.157

2.  Four amino acids define the CO2 binding pocket of enoyl-CoA carboxylases/reductases.

Authors:  Gabriele M M Stoffel; David Adrian Saez; Hasan DeMirci; Bastian Vögeli; Yashas Rao; Jan Zarzycki; Yasuo Yoshikuni; Soichi Wakatsuki; Esteban Vöhringer-Martinez; Tobias J Erb
Journal:  Proc Natl Acad Sci U S A       Date:  2019-06-26       Impact factor: 11.205

3.  Awakening the Sleeping Carboxylase Function of Enzymes: Engineering the Natural CO2-Binding Potential of Reductases.

Authors:  Iria Bernhardsgrütter; Kristina Schell; Dominik M Peter; Farshad Borjian; David Adrian Saez; Esteban Vöhringer-Martinez; Tobias J Erb
Journal:  J Am Chem Soc       Date:  2019-06-14       Impact factor: 15.419

4.  A catalytic dyad modulates conformational change in the CO2-fixing flavoenzyme 2-ketopropyl coenzyme M oxidoreductase/carboxylase.

Authors:  Jenna R Mattice; Krista A Shisler; Jennifer L DuBois; John W Peters; Brian Bothner
Journal:  J Biol Chem       Date:  2022-03-31       Impact factor: 5.486

  4 in total

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