Literature DB >> 27446978

Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons.

Hongmei Li1, Han-A Park1, Elizabeth A Jonas2.   

Abstract

This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons.

Entities:  

Year:  2014        PMID： 27446978      PMCID： PMC4950945          DOI： 10.21769/bioprotoc.1304

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  13 in total

1.  The presynaptic release apparatus is functional in the absence of dendritic contact and highly mobile within isolated axons.

Authors:  Stefan R Krueger; Annette Kolar; Reiko Maki Fitzsimonds
Journal:  Neuron       Date:  2003-12-04       Impact factor: 17.173

2.  Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical.

Authors:  Martin Wienisch; Jurgen Klingauf
Journal:  Nat Neurosci       Date:  2006-07-16       Impact factor: 24.884

3.  Postsynaptic excitability is necessary for strengthening of cortical sensory responses during experience-dependent development.

Authors:  Shoji Komai; Pawel Licznerski; Ali Cetin; Jack Waters; Winfried Denk; Michael Brecht; Pavel Osten
Journal:  Nat Neurosci       Date:  2006-08-20       Impact factor: 24.884

Review 4.  The efficiency of the synaptic vesicle cycle at central nervous system synapses.

Authors:  Tomás Fernández-Alfonso; Timothy A Ryan
Journal:  Trends Cell Biol       Date:  2006-07-12       Impact factor: 20.808

5.  High Ca2+-phosphate transfection efficiency in low-density neuronal cultures.

Authors:  Min Jiang; Gong Chen
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins.

Authors:  G Miesenböck; D A De Angelis; J E Rothman
Journal:  Nature       Date:  1998-07-09       Impact factor: 49.962

7.  Bcl-xL is necessary for neurite outgrowth in hippocampal neurons.

Authors:  Han-A Park; Pawel Licznerski; Kambiz N Alavian; Marya Shanabrough; Elizabeth A Jonas
Journal:  Antioxid Redox Signal       Date:  2015-01-10       Impact factor: 8.401

8.  Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons.

Authors:  Hongmei Li; Yingbei Chen; Adrienne F Jones; Richard H Sanger; Leon P Collis; Richard Flannery; Ewan C McNay; Tingxi Yu; Robert Schwarzenbacher; Blaise Bossy; Ella Bossy-Wetzel; Michael V L Bennett; Marc Pypaert; John A Hickman; Peter J S Smith; J Marie Hardwick; Elizabeth A Jonas
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-04       Impact factor: 11.205

9.  Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses.

Authors:  Björn Granseth; Benjamin Odermatt; Stephen J Royle; Leon Lagnado
Journal:  Neuron       Date:  2006-09-21       Impact factor: 17.173

10.  A Bcl-xL-Drp1 complex regulates synaptic vesicle membrane dynamics during endocytosis.

Authors:  Hongmei Li; Kambiz N Alavian; Emma Lazrove; Nabil Mehta; Adrienne Jones; Ping Zhang; Pawel Licznerski; Morven Graham; Takuma Uo; Junhua Guo; Christoph Rahner; Ronald S Duman; Richard S Morrison; Elizabeth A Jonas
Journal:  Nat Cell Biol       Date:  2013-06-23       Impact factor: 28.824
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