| Literature DB >> 27444329 |
N Pérez-Rodríguez1, C D Moreira2, A Torrado Agrasar3, J M Domínguez4.
Abstract
Ferulic acid esterases (FAE) were produced by Aspergillus terreus CECT 2808 from vine trimming shoots (VTS) and corn cob. Later, the fungal extracts thus obtained were used to enzymatically release ferulic acid (FA) from both substrates. Our findings showed a higher FAE activity in the enzymatic extracts produced on corn cob (0.070±0.004U/mL). Nevertheless, the enzymatic extracts produced on VTS demonstrated a better performance for FA release from both corn cob (2.05±0.01mg/g) and VTS (0.19±0.003mg/g). This result was probably because of the higher xylanase/FAE ratio determined in VTS extract. Therefore, an additional assay was carried out by supplementing corn cob extract with a commercial xylanase to test the influence of FAE/xylanase ratio in FA release. The results revealed the relevance of the FAE/xylanase ratio for an optimal FA release.Entities:
Keywords: Corn cob; Enzymatic hydrolysis; Ferulic acid; Feruloyl esterase; Vine trimming shoot
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Year: 2016 PMID： 27444329 DOI： 10.1016/j.enzmictec.2016.05.011
Source DB: PubMed Journal: Enzyme Microb Technol ISSN： 0141-0229 Impact factor: 3.493