Rubens Moreno de Freitas1, Cristiano Susin2,3, Wirla Maria da Silva Cunha Tamashiro4, João Antonio Chaves de Souza5, Claudio Marcantonio6, Ulf Me Wikesjö2,3, Luís Antônio Violin Dias Pereira7, Elcio Marcantonio6. 1. Department of Post-Graduation, ILAPEO - Latin American Institute of Dental Research and Education, Curitiba, PR, Brazil. 2. Laboratory for Applied Periodontal & Craniofacial Regeneration (LAPCR), Departments of Periodontics and Oral Biology, Dental College of Georgia, Augusta University, Augusta, GA, USA. 3. Department of Orthopaedic Surgery, Medical College of Georgia, Augusta University, Augusta, GA, USA. 4. Department of Genetics and Evolution and Bioagent, UNICAMP - State University of Campinas, Institute of Biology, Campinas, SP, Brazil. 5. Department of Stomatological Sciences - Periodontology, UFG - Federal University of Goias, Dental School, Goiania, GO, Brazil. 6. Department of Diagnostic and Surgery - Periodontics, UNESP - Univ Estadual Paulista, Araraquara Dental School, Araraquara, SP, Brazil. 7. Department of Biochemistry and Tissue Biology, UNICAMP - State University of Campinas, Institute of Biology, Campinas, SP, Brazil.
Abstract
AIM: The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) versus an autogenous bone graft (ABG). METHODS: Bone core biopsies from 24 subjects participating in a randomized clinical trial were obtained at dental implant placement, 6 months following alveolar ridge augmentation using rhBMP-2/ACS (rhBMP-2 at 1.5 mg/ml; total dose 4.2 mg) or a particulate ABG harvested from the mandibular retro-molar region. A titanium mesh was used to provide wound stability and space for bone formation. Analysis included histological/histometric observations and gene expression profile of the newly formed bone. RESULTS:rhBMP-2/ACS yielded bone marrow rich in capillaries, undifferentiated cells and bone lining cells compared with the ABG (p = 0.002). Whereas no significant differences were observed in total bone fraction (p = 0.53), non-vital bone particles trapped in lamellar vital bone were observed in the ABG group (p < 0.001). Real-time PCR showed greater BMP-2 and RUNX2 expression for rhBMP-2/ACS over the ABG (p = 0.001 and 0.0021, respectively), while the ABG exhibited greater expression of RANKL:OPG, BSP and OPN over rhBMP-2/ACS (p = 0.01, 0.005 and 0.0009, respectively). CONCLUSIONS: Our observations suggest that formative biological processes explain bone formation following implantation of rhBMP-2/ACS, whereas remodelling, resorptive/formative processes, characterizes sites receiving ABGs.
RCT Entities:
AIM: The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant humanbone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) versus an autogenous bone graft (ABG). METHODS: Bone core biopsies from 24 subjects participating in a randomized clinical trial were obtained at dental implant placement, 6 months following alveolar ridge augmentation using rhBMP-2/ACS (rhBMP-2 at 1.5 mg/ml; total dose 4.2 mg) or a particulate ABG harvested from the mandibular retro-molar region. A titanium mesh was used to provide wound stability and space for bone formation. Analysis included histological/histometric observations and gene expression profile of the newly formed bone. RESULTS:rhBMP-2/ACS yielded bone marrow rich in capillaries, undifferentiated cells and bone lining cells compared with the ABG (p = 0.002). Whereas no significant differences were observed in total bone fraction (p = 0.53), non-vital bone particles trapped in lamellar vital bone were observed in the ABG group (p < 0.001). Real-time PCR showed greater BMP-2 and RUNX2 expression for rhBMP-2/ACS over the ABG (p = 0.001 and 0.0021, respectively), while the ABG exhibited greater expression of RANKL:OPG, BSP and OPN over rhBMP-2/ACS (p = 0.01, 0.005 and 0.0009, respectively). CONCLUSIONS: Our observations suggest that formative biological processes explain bone formation following implantation of rhBMP-2/ACS, whereas remodelling, resorptive/formative processes, characterizes sites receiving ABGs.
Authors: Miriam Fussae Suzuki; João Ezequiel Oliveira; Renata Damiani; Eliana Rosa Lima; Kleicy Cavalcante Amaral; Anderson Maikon de Souza Santos; Geraldo Santana Magalhães; Leonardo Perez Faverani; Luis Antonio Violin Dias Pereira; Fabiana Medeiros Silva; Paolo Bartolini Journal: AMB Express Date: 2020-02-17 Impact factor: 3.298
Authors: João E Oliveira; Miriam F Suzuki; Renata Damiani; Eliana R Lima; Kleicy C Amaral; Anderson M S Santos; Geraldo S Magalhães; Leonardo P Faverani; Luís A V D Pereira; Paolo Bartolini Journal: Cells Date: 2021-12-14 Impact factor: 6.600