Literature DB >> 2743972

Preparation of plant DNA for separation by pulsed field gel electrophoresis.

K M Devos1, D Vercruysse-Dewitte.   

Abstract

A method was developed for the preparation of completely intact plant DNA embedded in agarose, and suitable for restriction enzyme digestion. Digestion with restriction enzyme was carried out according to modified protocols of Anand and Kenwrick et al. The new method of DNA isolation allows the separation of high molecular weight plant DNA by pulsed field gel electrophoresis.

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Year:  1989        PMID： 2743972     DOI： 10.1002/elps.1150100410

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  3 in total

1.  PFGE analysis of the rice genome: estimation of fragment sizes, organization of repetitive sequences and relationships between genetic and physical distances.

Authors:  K S Wu; S D Tanksley
Journal:  Plant Mol Biol       Date:  1993-10       Impact factor: 4.076

2.  Isolation of high-molecular-weight plant DNA for DNA damage quantitation: relative effects of solar 297 nm UVB and 365 nm radiation.

Authors:  F E Quaite; J C Sutherland; B M Sutherland
Journal:  Plant Mol Biol       Date:  1994-02       Impact factor: 4.076

3.  Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance.

Authors:  C Jung; M Kleine; E Fischer; R G Herrmann
Journal:  Theor Appl Genet       Date:  1990-05       Impact factor: 5.699
  3 in total

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