Nilufar Z Mamadalieva1, Khayrulla M Bobakulov1, Vittorio Vinciguerra2, Antonio Tiezzi2, Nasrulla D Abdullaev1, Lutfun Nahar3, Shahnoz S Azimova1, Satyajit D Sarker3. 1. Institute of the Chemistry of Plant Substances, Academy of Sciences, Mirzo Ulugbek str., 77, Tashkent, 100170, Uzbekistan. 2. Department for the Innovation in Biological, Agro-food and Forestal Systems, Tuscia University, Largo dell'Università blocco D, Via San Camillo de Lellis, 01100, Viterbo, Italy. 3. Medicinal Chemistry and Natural Products Research Group, School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, James Parsons Building, Byrom Street, Liverpool, L3 3AF, UK.
Abstract
INTRODUCTION: The genus Leonurus L. (fam: Lamiaceae) is represented in Uzbekistan by two species, L. panzerioides Popov. and L. turkestanicus V. I. Krecz. & Kuprian, which are used to treat nervous disorders and also as sedative and hypotensive agents. OBJECTIVES: To establish the taxonomic status of Leonurus panzerioides and L. turkestanicus based on their chemical constituents analysed by GC-MS and q-NMR. MATERIALS AND METHODS: Quantitative (1) H-NMR (q-NMR) was used to identify and quantify known major components in the methanol extracts of these two species. Additionally, the chemical composition of the essential oils obtained from the aerial parts of these plants were analysed by GC-MS. RESULTS: The q-NMR analyses of Leonurus panzerioides and L. turkestanicus revealed the presence of 8-acetylharpagide, harpagide, leonurine and stachydrine as major components. Using the GC-MS method, overall 24 and 39 constituents were identified, respectively, from L. panzerioides and L. turkestanicus oils. The major constituents of the essential oil of L. panzerioides were eugenol (30.9%) and p-vinyl guaiacol (15.8%), whereas thymol (40.1%) and octen-3-ol (13.1%) were the principal compounds in the essential oil of L. turkestanicus. CONCLUSION: The major components in Leonurus panzerioides and L. turkestanicus as identified by the GC-MS and q-NMR analyses, were similar to those present in other Leonurus species and thus provided chemotaxonomic evidence for the placement of these species under the genus Leonurus.
INTRODUCTION: The genus Leonurus L. (fam: Lamiaceae) is represented in Uzbekistan by two species, L. panzerioides Popov. and L. turkestanicus V. I. Krecz. & Kuprian, which are used to treat nervous disorders and also as sedative and hypotensive agents. OBJECTIVES: To establish the taxonomic status of Leonurus panzerioides and L. turkestanicus based on their chemical constituents analysed by GC-MS and q-NMR. MATERIALS AND METHODS: Quantitative (1) H-NMR (q-NMR) was used to identify and quantify known major components in the methanol extracts of these two species. Additionally, the chemical composition of the essential oils obtained from the aerial parts of these plants were analysed by GC-MS. RESULTS: The q-NMR analyses of Leonurus panzerioides and L. turkestanicus revealed the presence of 8-acetylharpagide, harpagide, leonurine and stachydrine as major components. Using the GC-MS method, overall 24 and 39 constituents were identified, respectively, from L. panzerioides and L. turkestanicus oils. The major constituents of the essential oil of L. panzerioides were eugenol (30.9%) and p-vinyl guaiacol (15.8%), whereas thymol (40.1%) and octen-3-ol (13.1%) were the principal compounds in the essential oil of L. turkestanicus. CONCLUSION: The major components in Leonurus panzerioides and L. turkestanicus as identified by the GC-MS and q-NMR analyses, were similar to those present in other Leonurus species and thus provided chemotaxonomic evidence for the placement of these species under the genus Leonurus.
Authors: Nilufar Z Mamadalieva; Davlat Kh Akramov; Elisa Ovidi; Antonio Tiezzi; Lutfun Nahar; Shahnoz S Azimova; Satyajit D Sarker Journal: Medicines (Basel) Date: 2017-02-10